User:Hemkersh

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	HUMAN:DUOX2	2014-04-03 10:29:02 CDT	GO:0016174 NAD(P)H oxidase activity (F)	PMID:21565790	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 1 shows that HeLa cells transfected with different expression vectors have different amounts of hydrogen peroxide generation based on. The mutant proteins produced significantly less hydrogen peroxide compared to wildtype protein.	challenge
acceptable	HUMAN:LYPA1	2014-04-04 15:25:37 CDT	GO:0016298 lipase activity (F)	PMID:24682756	ECO:0000314 direct assay evidence used in manual assertion	Figure 5 shows that treatment with an APT1 specific inhibitor, the laninamivir octanoate-hydrolyzing activity of the protein for peak II (APT1) is severely inhibited.	challenge
updatedbyinstructor	CANLF:CUBN	2014-04-04 20:19:16 CDT	GO:0016324 apical plasma membrane (C)	PMID:6321516	ECO:0000314 direct assay evidence used in manual assertion	Figure 7 shows that an antibody for the intrinsic factor-cobalamin complex (IF-cbl) [now known and cubam or cubilin and amnionless] is found at the microvillus pits of the apical membrane of ileum cells.	challenge
acceptable	HUMAN:AMNLS	2014-04-04 20:37:15 CDT	GO:0006898 receptor-mediated endocytosis (P)	PMID:20088845	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 4 shows that cells not expressing AMN cannot internalize intrinsic-factor-cobalamin into the cell through receptor-mediated endocytosis, while WT cells can	challenge
updatedbyinstructor	CANLF:AMNLS	2014-04-04 20:49:32 CDT	GO:0016324 apical plasma membrane (C)	PMID:15845892	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 5C uses immunoperoxidase staining to show that in wildtype dog kidney cells, amnionless and cubilin are localized to the plasma membrane, while in cells with mutant AMN, the protein complex is not localized there	challenge
unacceptable	?	2014-04-06 12:04:55 CDT	GO:0006898 receptor-mediated endocytosis (Figure 6 shows that MDCK cells with amnionless will work with mini-cubilin to endocytose)	PMID:15976000	IDA: Inferred from Direct Assay		challenge

acceptable:3
unacceptable:1
requires_changes:0
flagged:0

Annotations challenged by Hemkersh

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
unacceptable	Brookelle.christman, Team A5	STAA8:ARLS	GO:0009405 - obsolete pathogenesis (P)	PMID:10869073	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 1
unacceptable	Sprem, Team On a Boat	HUMAN:CRIM1	GO:0005010 - insulin-like growth factor receptor activity (F)	PMID:18669648	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 1

0 annotations fixed by Hemkersh

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