User:Enas.Alareef

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
unacceptable	LISMO:CSPB	2016-02-22 21:23:46 CST	GO:0043620 regulation of DNA-templated transcription in response to stress (P)	PMID:19151183	ECO:0000315 mutant phenotype evidence used in manual assertion	fig 2F Growth kinetics of ΔcspB, ΔcspD, and ΔcspBD at 4°C in DM. The results represent the means (± standard deviations) from duplicates of three independent experimental runs.	challenge
unacceptable	LISMN:A0A0E1R9H5	2016-03-03 10:29:04 CST	GO:0000163 protein phosphatase type 1 activity (F)	PMID:25841007	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig 3: Growth curves for Listeria monocytogenes and histidine kinase deletion mutant strains and the wild-type strain EGD-e in BHI broth at 3°C. All the strains were grown for 23 days, and the OD600 was measured every hour. The other histidine kinase mutant strains showed no significantly impaired growth compared to the wild type. (B) Growth curves for L. monocytogenes histidine kinase deletion mutant strains and the wild-type strain EGD-e in BHI broth at 37°C. . The data shown represent the mean OD600 values and standard deviations for five replicate cultures. The correspondence between the OD600 values and viable cell numbers was verified by plate counts.	challenge
updatedbyinstructor	LISMO:CSPB	2016-04-19 04:39:45 CDT	GO:0043488 regulation of mRNA stability (P)	PMID:23952475	ECO:0000316 genetic interaction evidence used in manual assertion	FIG. 3. Effect of cold shock proteins on hly mRNA stability as determined by quantitative reverse transcription–polymerase chain reaction. Total RNA was isolated from log phase EGDe wild-type and DcspABD cells at defined time intervals after addition of rifampicin.	challenge
unacceptable	LISIN:DPS	2016-04-15 10:08:09 CDT	GO:0033554 cellular response to stress (P)	PMID:10981685	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig4 showed a single 0.8-kb transcript hybridizing with the probe suggesting the existence of a monocistronic mRNA for flp gene expression. flp mRNA was detected at a significant level in absence of stress but after cold shock, a sharp increase of flp mRNA level was observed. Moreover, the abundance of the specific mRNA also increased significantly after heat shock suggesting that Flp could be a heat shock protein. These results strongly suggest that the L.monocytogenes LO28 Flp corresponds to the 19-kDa multi-stress protein	challenge

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unacceptable:3
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Annotations challenged by Enas.Alareef

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
unacceptable	Enas.Alareef, Team Alpha	LISMO:CSPB	GO:0043620 - obsolete regulation of DNA-templated transcription in response to stress (P)	PMID:19151183	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 6
unacceptable	Mitchell.L.Kennedy, Team Spartan	ENTFC:VANA	GO:0022607 - cellular component assembly (P)	PMID:26678143	ECO:0000317 genomic context evidence used in manual assertion	challenge	C: 4

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