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ENTFC:VANA
Contents
| Species (Taxon ID) | Enterococcus faecium (Streptococcus faecium). (1352) | |
| Gene Name(s) | vanA | |
| Protein Name(s) | Vancomycin/teicoplanin A-type resistance protein VanA
D-alanine--D-lactate ligase VanA ligase | |
| External Links | ||
| UniProt | P25051 | |
| EMBL | X56895 M97297 | |
| PIR | S12254 | |
| RefSeq | YP_001019035.1 YP_001974796.1 YP_002128399.1 YP_009073944.1 YP_009086373.1 YP_009090104.1 YP_976077.1 | |
| PDB | 1E4E | |
| PDBsum | 1E4E | |
| ProteinModelPortal | P25051 | |
| SMR | P25051 | |
| BindingDB | P25051 | |
| ChEMBL | CHEMBL6037 | |
| GeneID | 4670249 4783144 6385877 6779647 | |
| BioCyc | MetaCyc:MONOMER-15466 | |
| SABIO-RK | P25051 | |
| EvolutionaryTrace | P25051 | |
| GO | GO:0005737 GO:0005886 GO:0005524 GO:0008716 GO:0000287 GO:0030145 GO:0071555 GO:0009252 GO:0008360 GO:0046677 | |
| Gene3D | 3.30.1490.20 3.30.470.20 3.40.50.20 | |
| HAMAP | MF_00047 | |
| InterPro | IPR011761 IPR013815 IPR013816 IPR000291 IPR005905 IPR011095 IPR011127 IPR016185 | |
| PANTHER | PTHR23132 | |
| Pfam | PF07478 PF01820 | |
| SUPFAM | SSF52440 | |
| TIGRFAMs | TIGR01205 | |
| PROSITE | PS50975 PS00843 PS00844 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
| GO:0008716 |
D-alanine-D-alanine ligase activity |
ECO:0000314 |
F |
Fig. 2 is a TLC showing the D-alanine-D-alanine ligase activity of VanA. In the presence of VanA, the TLC shows that [14C]D-alanine is converted to [14C]D-alanine-D-alanine. In the absence of VanA, [14C]D-alanine does not form [14C]D-alanine-D-alanine. |
complete | |||||
| GO:0008716 |
D-alanine-D-alanine ligase activity |
ECO:0000314 |
F |
"FIGURE 2: Schcmatic figure of the proposed target modification mechanism involving VanA. MurF is a D-Ala-D-Ala adding enzyme; the cross-hatched circle represents an N-acetylmuramyl residue; the open circle represents an N-acetylglucosamine residue. The symbol inside the membrane represents the undecaprenyl lipid carrier. Depicted on the cell surface are the binding of peptidyl-D-Ala-D-Ala by vancomycin and the cross-linking reaction of a modified peptidoglycan terminus." |
complete | |||||
| GO:0022607 |
cellular component assembly |
ECO:0000317 |
|
P |
The vanA gene in Enterococcus faecium encodes for D-alanine D-lactate ligase which promotes ester bond formation producing a new peptidoglycan within the cell. This peptidoglycan inhibits vancomysin binding being it is a glycopeptide antibiotic. The GO ID shows the cellular component assembly pathaway which is what the formation of a new peptidoglycan is thus creating Vancomycin resistance. The Pubmed article shows the study showing that vanA has been proven to contain antibiotic resistant properties in animals and when included in our diet, further promotes antibiotic resistant bacterial strains in humans |
complete | ||||
| GO:0008716 |
D-alanine-D-alanine ligase activity |
ECO:0000314 |
F |
VanA is a ligase that binds D-Ala with D-Ala |
complete | |||||
|
enables |
GO:0008716 |
D-alanine-D-alanine ligase activity |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0005524 |
ATP binding |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005737 |
cytoplasm |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
enables |
GO:0008716 |
D-alanine-D-alanine ligase activity |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0046872 |
metal ion binding |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005737 |
cytoplasm |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
UniRule:UR000001246 |
C |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0009252 |
peptidoglycan biosynthetic process |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
UniRule:UR000001246 |
P |
Seeded From UniProt |
complete | ||
|
enables |
GO:0008716 |
D-alanine-D-alanine ligase activity |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
UniRule:UR000001246 |
F |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0071555 |
cell wall organization |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0016874 |
ligase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0000166 |
nucleotide binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0009252 |
peptidoglycan biosynthetic process |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0005524 |
ATP binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0046677 |
response to antibiotic |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0046872 |
metal ion binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005886 |
plasma membrane |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0008360 |
regulation of cell shape |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0016020 |
membrane |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ 1.0 1.1 Bugg, TD et al. (1991) Molecular basis for vancomycin resistance in Enterococcus faecium BM4147: biosynthesis of a depsipeptide peptidoglycan precursor by vancomycin resistance proteins VanH and VanA. Biochemistry 30 10408-15 PubMed GONUTS page
- ↑ Bugg, TD et al. (1991) Identification of vancomycin resistance protein VanA as a D-alanine:D-alanine ligase of altered substrate specificity. Biochemistry 30 2017-21 PubMed GONUTS page
- ↑ Guerrero-Ramos, E et al. (2016) Antimicrobial resistance and virulence genes in enterococci from wild game meat in Spain. Food Microbiol. 53 156-64 PubMed GONUTS page
- ↑ Arthur, M et al. (1997) The VanS sensor negatively controls VanR-mediated transcriptional activation of glycopeptide resistance genes of Tn1546 and related elements in the absence of induction. J. Bacteriol. 179 97-106 PubMed GONUTS page
