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PMID:10981685
| Citation |
Hébraud, M and Guzzo, J (2000) The main cold shock protein of Listeria monocytogenes belongs to the family of ferritin-like proteins. FEMS Microbiol. Lett. 190:29-34 |
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| Abstract |
The transfer of the food-borne pathogen Listeria monocytogenes from 30 to 5 degrees C was characterized by the sharp induction of a low molecular mass protein. This major cold shock protein has an isoelectric point at pH 5.1 and a molecular mass of about 18 kDa, as observed on two-dimensional gel electrophoresis (2-DE) pattern. Its N-terminal sequence, obtained from the 2-DE spot, shared a complete sequence identity with a Listeria innocua non-heme iron-binding ferritin. The purification of these ferritin-like proteins (Flp) revealed a native molecular mass of about 100-110 kDa which indicates a polypeptide composed of six 18 kDa-subunits. Northern analysis indicated the presence of a 0.8-kb monocistronic mRNA in exponential growing cells and an important increase inflp mRNA amount after a downshift but also an upshift in temperature. |
| Links | |
| Keywords |
Bacterial Proteins/biosynthesis; Bacterial Proteins/chemistry; Bacterial Proteins/classification; Bacterial Proteins/genetics; Cold Temperature; Electrophoresis, Polyacrylamide Gel/methods; Ferritins/biosynthesis; Ferritins/chemistry; Ferritins/classification; Ferritins/genetics; Heat-Shock Response; Humans; Listeria monocytogenes/genetics; Listeria monocytogenes/growth & development; Listeria monocytogenes/metabolism; Molecular Sequence Data; RNA, Bacterial/genetics; RNA, Bacterial/metabolism; RNA, Messenger/genetics; RNA, Messenger/metabolism; Temperature; Transcription, Genetic |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0033554: cellular response to stress |
ECO:0000315: |
P |
Fig4 showed a single 0.8-kb transcript hybridizing with the probe suggesting the existence of a monocistronic mRNA for flp gene expression. flp mRNA was detected at a significant level in absence of stress but after cold shock, a sharp increase of flp mRNA level was observed. Moreover, the abundance of the specific mRNA also increased significantly after heat shock suggesting that Flp could be a heat shock protein. These results strongly suggest that the L.monocytogenes LO28 Flp corresponds to the 19-kDa multi-stress protein |
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Notes
See also
References
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