PMID:25841007

Citation	Pöntinen, A, Markkula, A, Lindström, M and Korkeala, H (2015) Two-Component-System Histidine Kinases Involved in Growth of Listeria monocytogenes EGD-e at Low Temperatures. Appl. Environ. Microbiol. 81:3994-4004
Abstract	Two-component systems (TCSs) aid bacteria in adapting to a wide variety of stress conditions. While the role of TCS response regulators in the cold tolerance of the psychrotrophic foodborne pathogen Listeria monocytogenes has been demonstrated previously, no comprehensive studies showing the role of TCS histidine kinases of L. monocytogenes at low temperature have been performed. We compared the expression levels of each histidine kinase-encoding gene of L. monocytogenes EGD-e in logarithmic growth phase at 3°C and 37°C, as well as the expression levels 30 min, 3 h, and 7 h after cold shock at 5°C and preceding cold shock (at 37°C). We constructed a deletion mutation in each TCS histidine kinase gene, monitored the growth of the EGD-e wild-type and mutant strains at 3°C and 37°C, and measured the minimum growth temperature of each strain. Two genes, yycG and lisK, proved significant in regard to induced relative expression levels under cold conditions and cold-sensitive mutant phenotypes. Moreover, the ΔresE mutant showed a lower growth rate than that of wild-type EGD-e at 3°C. Eleven other genes showed upregulated gene expression but revealed no cold-sensitive phenotypes. The results show that the histidine kinases encoded by yycG and lisK are important for the growth and adaptation of L. monocytogenes EGD-e at low temperature.
Links	PubMed PMC4524140 Online version:10.1128/AEM.00626-15
Keywords	Adaptation, Physiological/genetics; Cold Temperature; Gene Expression Regulation, Bacterial; Listeria monocytogenes/enzymology; Listeria monocytogenes/genetics; Listeria monocytogenes/growth & development; Protein Kinases/genetics; Protein Kinases/metabolism; Real-Time Polymerase Chain Reaction; Sequence Deletion
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
LISMN:A0A0E1R9H5		GO:0000163: protein phosphatase type 1 activity	ECO:0000315:		F		Fig 3: Growth curves for Listeria monocytogenes and histidine kinase deletion mutant strains and the wild-type strain EGD-e in BHI broth at 3°C. All the strains were grown for 23 days, and the OD600 was measured every hour. The other histidine kinase mutant strains showed no significantly impaired growth compared to the wild type. (B) Growth curves for L. monocytogenes histidine kinase deletion mutant strains and the wild-type strain EGD-e in BHI broth at 37°C. . The data shown represent the mean OD600 values and standard deviations for five replicate cultures. The correspondence between the OD600 values and viable cell numbers was verified by plate counts.	complete CACAO 11347

References

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