PMID:23952475

Citation	Schärer, K, Stephan, R and Tasara, T (2013) Cold shock proteins contribute to the regulation of listeriolysin O production in Listeria monocytogenes. Foodborne Pathog. Dis. 10:1023-9
Abstract	Cold shock proteins (Csps) are multifunctional nucleic acid binding proteins used to regulate a wide range of gene expression responses in bacteria. We report here that Csps regulate the production of the pore-forming cytolysin listeriolysin (LLO) and hemolysis phenotypes in Listeria monocytogenes. A triple csp gene deletion mutant incapable of producing any Csps, as well as double csp gene deletion mutants only producing either CspA or CspD, caused less hemolysis and produced lower LLO concentration. On the other hand, another double csp gene deletion mutant that produces CspB retained hemolysis and LLO production levels that are similar to the parental wild-type strain. Transcription analysis showed that in absence of all three csp genes or cspB alone, L. monocytogenes cells have decreased levels of hly gene transcripts, which code for the synthesis of LLO proteins. A comparative examination of mRNA stability showed that hly transcripts were more rapidly degraded in L. monocytogenes triple csp gene deletion mutant cells that are not capable of producing Csps. Overall, our results indicate that Csps, in particular CspB, are important components of gene expression regulatory mechanisms that promote efficient LLO production and hence virulence responses of L. monocytogenes.
Links	PubMed Online version:10.1089/fpd.2013.1562
Keywords	Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Bacterial Toxins/genetics; Bacterial Toxins/metabolism; Cold Shock Proteins and Peptides/genetics; Cold Shock Proteins and Peptides/metabolism; Gene Deletion; Gene Expression Regulation, Bacterial; Heat-Shock Proteins/genetics; Heat-Shock Proteins/metabolism; Hemolysin Proteins/genetics; Hemolysin Proteins/metabolism; Hemolysis; Listeria monocytogenes/genetics; Listeria monocytogenes/pathogenicity; Listeria monocytogenes/physiology; Listeriosis; Mutation; Phenotype; RNA Stability; RNA, Messenger/genetics; RNA, Messenger/metabolism; Virulence; Virulence Factors
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
LISMO:CSPA		GO:0048255: mRNA stabilization	ECO:0000316: genetic interaction evidence used in manual assertion	UniProtKB:P0A357 UniProtKB:Q92AD0	P		Fig. 1 shows difference in hemolysis in the wild-type and cspABD L. monocytogenes species. GO term for the original annotation has been made obsolete. DAS replaced it withGO:0048255. See talk page for details.	complete CACAO 11279
LISMO:CSPB		GO:0043488: regulation of mRNA stability	ECO:0000316:	UniProtKB:P0A355 UniProtKB:Q92AD0	P		FIG. 3. Effect of cold shock proteins on hly mRNA stability as determined by quantitative reverse transcription–polymerase chain reaction. Total RNA was isolated from log phase EGDe wild-type and DcspABD cells at defined time intervals after addition of rifampicin.	complete CACAO 11520
LISIN:CSPB		GO:0045893: positive regulation of transcription, DNA-templated	ECO:0000316:	UniProtKB:Q7ANX6	P		Figure 2. This indicated CspB's role in the regulation of hly mRNA transcription and Listerolysin O production.	complete CACAO 11316

References

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