My Annotations
| Status | Page | Date/Time | GO Term (Aspect) | Reference | Evidence | Notes | Links |
|---|
| updatedbyinstructor | EBVB9:BALF1 | 2013-03-30 19:32:55 CDT | GO:0043066 negative regulation of apoptotic process (P) | PMID:22230317 | ECO:0000314 direct assay evidence used in manual assertion | Figure 4 presents the role of BALF1 in regulating apoptosis. After being placed in an environment to cause serum starvation-induced apoptosis, the control pcDNA transfectants showed a statistically significant higher number of apoptotic control cells in comparison to BALF1 transfectants (4A). Part 4B shows 38.9% of apoptotic pcDNA-transfectants, which was about 4-10 fold increase from BALF1-transfectants. Figure 4C represents that the BALF1 expressing cells showed a much higher survival rate in serum starvation conditions which seems to be attributable to suppression of apoptosis.
| challenge |
| updatedbyinstructor | EBVA8:BARF1 | 2013-04-09 12:04:21 CDT | GO:0051781 positive regulation of cell division (P) | PMID:23458996 | ECO:0000314 direct assay evidence used in manual assertion | Figure 2A directly displays the results of BARF1-trasnfected HaCaT cell growth over 16 days in comparison to vector alone-transfected cells. BARF1 is a secreted protein that can be a powerful mitogen. In the graph shown in figure 2A, three BARF1-transfected cell cultures display more than a two-fold increase in cell division compared to vector-transfected and vector free cell cultures. Figure 2B displays the presence of the BARF1 fusion peptide of the protein in the BARF1-transfectant cells which is not present in vector-transfectant cells. This figure demonstrates the role BARF1 plays in positively regulating cell division.
| challenge |
| acceptable | HHV11:US9 | 2013-04-14 02:15:57 CDT | GO:0075733 intracellular transport of viral material (P) | PMID:23322573 | ECO:0000315 mutant phenotype evidence used in manual assertion | In neurotropic strains of the herpes simplex virus (HSV), DNA is transported from the neuron body to the axon terminus. The protein Us9 is involved in the regulation of the genome transport. Figure 2 compares the percentage of transported DNA in cells containing wild type Us9 protein, and two other cells containing different Us9 gene mutations. Figure 2A demonstrates the significant effect the Us9 mutation has on DNA transport on day 3,4, and 5. Figure 2B, a different strain of HSV, demonstrates the significant effect the mutated Us9 protein has on genome transport as well, although the results were only statistically significant for day 3. None the less, the figure demonstrates that Us9 is significantly involved in the transport of the viral genome in neurotropic strains of HSV.
| challenge |
| acceptable | CVHOC:HEMA | 2013-04-14 13:21:07 CDT | GO:0019058 viral infectious cycle (P) | PMID:23283955 | ECO:0000315 mutant phenotype evidence used in manual assertion | HE in human coronavirus plays a significant role in the production of an infectious virus, even though the S protein is the main viral factor in coronavirus infection. Figure 4 demonstrates the significant role HE also plays in the production of an infectious viral product. When a functional HE was added to the mutated transfectant, more viral antigens were produced. Figure 4C demonstrates the larger amount of viral antigens present in the transfectants with a functional HE compared to the pcDNA transfectant. Figure 4D demonstrates the significant amount of viral antigens who originally had a mutated HE protein were transfected with a functional HE protein. Therefore, it is apparent that the HE protein plays a significant role in producing infectious viruses.
| challenge |
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