PMID:23458996

Citation	Sakka, E, Hausen, AZ, Houali, K, Liu, H, Fiorini, S and Ooka, T' (2013) Cellular localization of BARF1 oncoprotein and its cell stimulating activity in human epithelial cell. Virus Res. '
Abstract	BARF1 gene encoded by Epstein-Barr virus is capable of immortalizing the primary monkey epithelial cells and of inducing malignant transformation in human EBV-negative B cell lines as well as rodent fibroblast. This oncoprotein is a secreted protein capable of acting as a powerful mitogen. We have studied the effect of BARF1 protein in transfected or BARF1 protein treated human HaCaT epithelial cells. In BARF1-transfected cells, cell growth was activated and its protein was found both in culture medium and cellular compartment (membrane, cytoplasm and nuclei). When purified BARF1 protein was exogenously added in the cell culture medium of HaCaT cells in absence of fetal calf serum led to its entrance into cells and its intracellular localization in cytoplasm, nuclear periphery and nuclei at 14h treatment, determined by confocal and immunoelectron microscopy. Cell fractionation confirmed its nuclear localization. Nuclear localization was observed in both systems. More interestingly, purified BARF1 protein p29 exogenously added in the cell culture medium activated cell passage of G1 to S phase. S phase activation by its autocrine activity and its tumorigenic activity would be associated with the development of EBV-associated carcinomas.
Links	PubMed Online version:10.1016/j.virusres.2013.01.016
Keywords
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
EBVA8:BARF1		GO:0051781: positive regulation of cell division	ECO:0000314:		P		Figure 2A directly displays the results of BARF1-trasnfected HaCaT cell growth over 16 days in comparison to vector alone-transfected cells. BARF1 is a secreted protein that can be a powerful mitogen. In the graph shown in figure 2A, three BARF1-transfected cell cultures display more than a two-fold increase in cell division compared to vector-transfected and vector free cell cultures. Figure 2B displays the presence of the BARF1 fusion peptide of the protein in the BARF1-transfectant cells which is not present in vector-transfectant cells. This figure demonstrates the role BARF1 plays in positively regulating cell division.	complete CACAO 7429
EBVA8:BARF1	involved_in	GO:0051781: positive regulation of cell division	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete

References

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