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PMID:23322573
| Citation |
Draper, JM, Huang, G, Stephenson, GS, Bertke, AS, Cortez, DA and LaVail, JH (2013) Delivery of herpes simplex virus to retinal ganglion cell axon is dependent on viral protein Us9. Invest. Ophthalmol. Vis. Sci. 54:962-7 |
|---|---|
| Abstract |
How herpes simplex virus (HSV) is transported from the infected neuron cell body to the axon terminal is poorly understood. Several viral proteins are candidates for regulating the process, but the evidence is controversial. We compared the results of Us9 deletions in two HSV strains (F and NS) using a novel quantitative assay to test the hypothesis that the viral protein Us9 regulates the delivery of viral DNA to the distal axon of retinal ganglion cells in vivo. We also deleted a nine-amino acid motif in the Us9 protein of F strain (Us9-30) to define the role of this domain in DNA delivery. |
| Links |
PubMed PMC3564453 Online version:10.1167/iovs.12-11274 |
| Keywords |
Animals; Axons/metabolism; Axons/pathology; Axons/virology; Cell Line; DNA, Viral/genetics; Disease Models, Animal; Eye Infections, Viral/genetics; Eye Infections, Viral/pathology; Eye Infections, Viral/virology; Gene Expression Regulation, Viral; Genome, Viral; Male; Mice; Mice, Inbred BALB C; Polymerase Chain Reaction; Retinal Ganglion Cells/metabolism; Retinal Ganglion Cells/pathology; Retinal Ganglion Cells/virology; Simplexvirus/genetics; Viral Envelope Proteins/biosynthesis; Viral Envelope Proteins/genetics; Viral Proteins/genetics; Viral Proteins/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
involved_in |
GO:0075733: intracellular transport of virus |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
| GO:0075733: intracellular transport of viral material |
ECO:0000315: |
P |
In neurotropic strains of the herpes simplex virus (HSV), DNA is transported from the neuron body to the axon terminus. The protein Us9 is involved in the regulation of the genome transport. Figure 2 compares the percentage of transported DNA in cells containing wild type Us9 protein, and two other cells containing different Us9 gene mutations. Figure 2A demonstrates the significant effect the Us9 mutation has on DNA transport on day 3,4, and 5. Figure 2B, a different strain of HSV, demonstrates the significant effect the mutated Us9 protein has on genome transport as well, although the results were only statistically significant for day 3. None the less, the figure demonstrates that Us9 is significantly involved in the transport of the viral genome in neurotropic strains of HSV. |
complete | ||||
See also
References
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