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User:Barnesgr

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SUPER BOWL (Round 5)

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableSTAAU:BLAI2012-04-14 14:47:52 CDTGO:0010468 regulation of gene expression (P)PMID:8517704ECO:0000314 direct assay evidence used in manual assertion

Fig. 2 shows that expression PBP 2a and beta-lactamase was repressed in cells transformed with blaI, but PBP 2a and beta-lactamase were expressed constitutively in untransformed cells. Furthermore, expression in transformed cells could be induced.

challenge
requireschangesSTAAU:MECI2012-04-14 15:28:01 CDTGO:0006355 regulation of transcription, DNA-dependent (P)PMID:9555900ECO:0000314 direct assay evidence used in manual assertion

Table 2 shows that in cells transformed with mecI, the beta-galactosidase activity of mecA-lacZ transcriptional fusion can be induced with the presence of CBAP. Furthermore, it is shown in Fig. 2 that mecI binds to a region of DNA between the mecA and mecR1 genes and protects it from DNase.

challenge
requireschangesSTAA1:MECA2012-04-14 22:37:58 CDTGO:0046677 response to antibiotic (P)PMID:2708325ECO:0000314 direct assay evidence used in manual assertion

Fig. 2 shows the production of penicillin binding protein (PBP) in strains of Staphylococcus aureus transformed with pSP53 with the mecA gene.

challenge
requireschangesENTFC:VANZ2012-04-15 00:27:10 CDTGO:0046677 response to antibiotic (P)PMID:7867956ECO:0000314 direct assay evidence used in manual assertion

Table 2 shows that cells with plasmids containing the vanZ gene have a higher minimum inhibitory concentration of teicoplanin than cells without the vanZ gene.

challenge
requireschangesENTFC:A1JV202012-04-15 00:39:21 CDTGO:0046677 response to antibiotic (P)PMID:7867956ECO:0000314 direct assay evidence used in manual assertion

Table 2 shows that cells transformed with plasmids containing vanY have higher minimum inhibitory concentrations (MIC) of vancomycin than cells without vanY.

challenge
unacceptableENTFC:Q9EZQ92012-04-15 14:25:01 CDTGO:0046677 response to antibiotic (P)PMID:15388450ECO:0000314 direct assay evidence used in manual assertion

Table 2 shows that enterococci with VanD have high minimum inhibitory concentrations (MIC) of vancomycin.

challenge
requireschangesENTFC:Q9EZQ92012-04-15 14:27:49 CDTGO:0016805 dipeptidase activity (F)PMID:15388450ECO:0000314 direct assay evidence used in manual assertion

Table 6 shows the dipeptidase activity of enterococci with VanD.

challenge

acceptable:1
unacceptable:1
requires_changes:5
flagged:0

Annotations challenged by Barnesgr

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history

0 annotations fixed by Barnesgr


MSU Internal

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableENTFC:VANA2012-03-29 11:59:25 CDTGO:0008716 D-alanine-D-alanine ligase activity (F)PMID:1931965ECO:0000314 direct assay evidence used in manual assertion

Fig. 2 is a TLC showing the D-alanine-D-alanine ligase activity of VanA. In the presence of VanA, the TLC shows that [14C]D-alanine is converted to [14C]D-alanine-D-alanine. In the absence of VanA, [14C]D-alanine does not form [14C]D-alanine-D-alanine.

challenge

acceptable:1
unacceptable:0
requires_changes:0
flagged:0

Annotations challenged by Barnesgr

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history

0 annotations fixed by Barnesgr