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PMID:8517704
| Citation |
Hackbarth, CJ and Chambers, HF (1993) blaI and blaR1 regulate beta-lactamase and PBP 2a production in methicillin-resistant Staphylococcus aureus. Antimicrob. Agents Chemother. 37:1144-9 |
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| Abstract |
For Staphylococcus aureus, it is hypothesized that two genes located upstream of the beta-lactamase gene, blaZ, are required for the inducible expression of beta-lactamase. blaR1 is predicted to encode a signal-transducing membrane protein, and blaI is predicted to encode a repressor protein. These same two genes may also regulate the production of penicillin-binding protein 2a (PBP 2a), a protein essential for expression of methicillin resistance. To confirm that these two genes encode products that can control both beta-lactamase and PBP 2a production, blaI, blaR1, and blaZ with a 150-nucleotide deletion at the 3' end were subcloned from a 30-kb staphylococcal beta-lactamase plasmid and three beta-lactamase-negative strains of methicillin-resistant S. aureus were transformed with the recombinant plasmid containing that insert. The production of PBP 2a and a nonfunctional beta-lactamase was detected by fluorography and by immunoblots with polyclonal antisera directed against each of the proteins. Whereas the parent strains did not produce beta-lactamase and constitutively produced PBP 2a, PBP 2a and a truncated beta-lactamase were now inducible in the transformants. Therefore, two plasmid-derived genes regulate the production of both PBP 2a and beta-lactamase. |
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| Keywords |
Bacterial Proteins/biosynthesis; Bacterial Proteins/genetics; Base Sequence; Blotting, Southern; Carrier Proteins/biosynthesis; Carrier Proteins/genetics; Carrier Proteins/immunology; Cloning, Molecular; Enzyme Induction; Escherichia coli/drug effects; Escherichia coli/genetics; Hexosyltransferases; Immune Sera; Metalloendopeptidases/biosynthesis; Metalloendopeptidases/genetics; Methicillin Resistance; Molecular Sequence Data; Muramoylpentapeptide Carboxypeptidase/biosynthesis; Muramoylpentapeptide Carboxypeptidase/genetics; Muramoylpentapeptide Carboxypeptidase/immunology; Nucleic Acid Hybridization; Penicillin-Binding Proteins; Peptidyl Transferases; Plasmids; Staphylococcus aureus/drug effects; Staphylococcus aureus/genetics; Transformation, Bacterial; beta-Lactamases/biosynthesis; beta-Lactamases/genetics; beta-Lactamases/immunology |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
involved_in |
GO:0010468: regulation of gene expression |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
| GO:0010468: regulation of gene expression |
ECO:0000314: |
P |
Fig. 2 shows that expression PBP 2a and beta-lactamase was repressed in cells transformed with blaI, but PBP 2a and beta-lactamase were expressed constitutively in untransformed cells. Furthermore, expression in transformed cells could be induced. |
complete | ||||
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