GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
Cacao
| GO:0071236 | cellular response to antibiotic | PMID:24412198 | IDA: Inferred from Direct Assay | P | ||||
| This annotation made on page: ECOLI:LAMB By: Fclingen (group Team What the Fox Says) on 2014-01-28 20:19:27 CST. | ||||||||
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| Entry Type | Challenging User,Group | Time/Date | Challenge Reason | Points/Assessment |
|---|---|---|---|---|
| Challenge | Rausch14, MichSt14A 18 | 2014-04-10 10:47:11 CDT | I would use the GO term GO:0015288 (porin activity). The evidence code should be IMP (inferred from mutant phenotype). Figure 2 is evidence for porin activity: mutations to the lamB gene result in greater live cell density when grown in the presence of antibiotics. Strains with non-mutant porin activity typically have lower cell densities. When lamB activity is disrupted uptake of antibiotics is not a great since porin activity is disrupted. | 4 |
| Challenge | Rausch14, MichSt14A 18 | 2014-04-10 10:08:45 CDT | I believe figure 2 is better for demonstrating the a cellular response to different antibiotics when the lamB gene has been mutated. This figure compares the survival rates of cell lines grown with antibiotics at specific times. The evidence code should be changed to inferred from mutant phenotype (IMP). | 0 |
| Challenge | Malexander2, Team Pancakes At Nite | 2014-02-08 12:23:46 CST | Evidence Code should be something like this EXP: Inferred from Experiment or possibly this IPI: Inferred from Physical Interaction | 0 |
| Challenge | Kfree, Team Bfast Taco | 2014-02-03 15:25:42 CST | GO term includes a response to term which is illegal. GO:0015288 porin activity may be more appropriate. LamB was shown through a spectrofluorometry assay to serve as a porin to influx the antibiotic. Strains with greater antibiotic resistance have lower expression of LamB because it facilitates the transfer of the antibiotic through porin activity. | 2 |
| Private Assessment | Suzialeksander | 2014-05-06 16:38:09 CDT | You need to be an instructor to view these notes. | Updated by Instructor |
| Private Assessment | JimHu | 2014-04-15 12:21:42 CDT | You need to be an instructor to view these notes. | Requires Changes ✔ Protein ✔ Publication ✔ Qualifier ✔ Go term ✔ Evidence ✔ With/From ✗ Notes ✔ Unique/Original |
| Public Assessment | DanielRenfro | 2014-04-10 10:47:11 CDT | This annotation has been flagged because it has been edited since last assessment ||GO:0071236||cellular response to antibiotic||PMID:24412198||IDA: Inferred from Direct Assay||||P||Figure 3. LamB is decreased in antibiotic resistant strains.||complete | Flagged |
| Public Assessment | DanielRenfro | 2014-04-10 10:08:45 CDT | This annotation has been flagged because it has been edited since last assessment ||GO:0071236||cellular response to antibiotic||PMID:24412198||IDA: Inferred from Direct Assay||||P||Figure 3. LamB is decreased in antibiotic resistant strains.||complete | Flagged |
| Private Assessment | Suzialeksander | 2014-02-10 11:44:06 CST | You need to be an instructor to view these notes. | Requires Changes |
| Public Assessment | DanielRenfro | 2014-02-08 12:23:46 CST | This annotation has been flagged because it has been edited since last assessment | Flagged |
| Private Assessment | Suzialeksander | 2014-02-04 15:56:54 CST | You need to be an instructor to view these notes. | Requires Changes ✔ Protein ✔ Publication ✔ Qualifier ✗ Go term ✗ Evidence ✔ With/From ✗ Notes ✔ Unique/Original |
