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Category:Team Master BLASTers

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SUPER BOWL ROUND Spring 2012

StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
requireschangesMOUSE:IZUM1Watzadon, Team Master BLASTers2012-04-15 15:00:43 CDTGO:0007342 fusion of sperm to egg plasma membrane (P)PMID:15759005ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3 shows male infertility when Izumo gene is disrupted with insertional mutagensis

challenge
requireschangesCAEEL:SPE9Watzadon, Team Master BLASTers2012-04-15 14:53:36 CDTGO:0009566 fertilization (P)PMID:9546393ECO:0000315 mutant phenotype evidence used in manual assertion

This paper describes mutant SPE-9 deficient nematode spermatozoa that were unable to fertilize normal egg cells preventing egg fertilization. Figure 1 shows a graphical representation of the infertility and ovulation of SPE-9 mutant nematodes. Figure 2 and Figure 3 show microscope images of mutant spermatozoa cells

challenge
requireschangesRABIT:SP17Watzadon, Team Master BLASTers2012-04-15 14:36:56 CDTGO:0007339 binding of sperm to zona pellucida (P)PMID:7525387ECO:0000314 direct assay evidence used in manual assertion

Figure 10 shows the binding of the sperm sp17 protein to the to zona pellucida region of egg cells

challenge
requireschangesHALRO:SPRMWatzadon, Team Master BLASTers2012-04-15 14:12:04 CDTGO:0008233 peptidase activity (F)PMID:6365918ECO:0000314 direct assay evidence used in manual assertion

This paper alongside Figure 2 and Figure 3 describe the stability and ability of this enzyme to cleave the peptide sequence Val-Pro-Arg

challenge
requireschangesAPLCA:NADAWatzadon, Team Master BLASTers2012-04-15 13:56:05 CDTGO:0003953 NAD+ nucleosidase activity (F)PMID:1650254ECO:0000314 direct assay evidence used in manual assertion

Figure 2 shows the direct assay of the extracted and purified protein and its NADase activity when nicotinamide is present. Figure 3 shows the enzymatic kinetics of this enzyme and its product formation.

challenge
requireschangesHALRU:ELYSWatzadon, Team Master BLASTers2012-04-15 13:43:03 CDTGO:0009566 fertilization (P)PMID:2377618ECO:0000314 direct assay evidence used in manual assertion

Figure 1 shows the The species specificity of red and pink abalone lysins to dissolve isolated 125I-labeled VE, demonstrating the lysin activity of these proteins

challenge
requireschangesLEIMA:Q5C8V6Nguyenka, Team Master BLASTers2012-04-15 10:27:48 CDTGO:0003333 amino acid transmembrane transport (P)PMID:15632173ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3B - The gene encoding for LmPOT1 was cloned in the pHD309 vector enabling the integration of LmPOT1 into T. brucei, an organism related to L. major which has a low putrescine uptake. A control was made by inserting the empty vector into T. brucei. Putrescine transport was measured in both strains. The LmPOT1 T. brucei strain showed a significant ability to uptake putrescine while the control's putrescine uptake was negligible.

challenge
requireschangesLEIMA:Q7K9G0Nguyenka, Team Master BLASTers2012-04-15 01:05:41 CDTGO:0016784 3-mercaptopyruvate sulfurtransferase activity (F)PMID:12419809ECO:0000314 direct assay evidence used in manual assertion

Figure 3B - Western blot analysis of the expression of 3-mercaptopyruvate sulfurtransferase (MST) when L. major cells were exposed to hypo-sulfur stress. Lane 1 contains the control while lane 2 contains cells exposed hypo-sulfur stress. Exposed L. major cells responded to hypo-sulfur stress over 24 h by increased expression of MST.

challenge
requireschangesLEIMA:HSP70Nguyenka, Team Master BLASTers2012-04-14 22:48:53 CDTGO:0006950 response to stress (P)PMID:15544167ECO:0000314 direct assay evidence used in manual assertion

Figure 3C-3E - Western blot analysis of HSP70 expression on shock with potassium antimonyl tartrate (SbIII) over time. These figures clearly demonstrate that HSP70 expression increases in Leishmania cells when exposed to SbIII.

challenge


==MSU Internal Competition==

StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
unacceptable?Watzadon, Team Master BLASTers2012-04-01 16:00:30 CDT GO:0004474 (Figure 4 and Figure 5 show the effects of varying glyoxylate(A) and succinate (B) on the isocitrate lyase condensation reaction of the D475N mutant of the Euglena gracilis glyoxylate cycle enzyme)malate synthase activityIDA: Inferred from Direct Assay
challenge
requireschangesXENLA:E7FLL1Nguyenka, Team Master BLASTers2012-03-31 14:25:44 CDTGO:0070188 Stn1-Ten1 complex (C)PMID:22086929ECO:0000314 direct assay evidence used in manual assertion

Figure 2 - assay shows that protein complex binds to ssDNA

Figure 5 - assay shows that xStn1 immunodepletion does not affect DNA polymerase α-primase binding to ssDNA

challenge
requireschangesXENLA:EF1A2Nguyenka, Team Master BLASTers2012-03-28 10:14:23 CDTGO:0008568 microtubule-severing ATPase activity (F)PMID:7939665ECO:0000314 direct assay evidence used in manual assertion

Fig. 3 depicts the lack of microtubule-severing activity in an immunodepletion experiment when the amount of EF-1alpha was reduced ~70%.

challenge
acceptableHUMAN:SSFA2Watzadon, Team Master BLASTers2012-03-27 11:54:38 CDTGO:0051015 actin filament binding (F)PMID:14673706ECO:0000314 direct assay evidence used in manual assertion

Figure 4: co-localization of KRAP (homo sapien) with actin filaments using florescence antibodies.

challenge

Pages in category "Team Master BLASTers"

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