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PMID:14673706
| Citation |
Inokuchi, J, Komiya, M, Baba, I, Naito, S, Sasazuki, T and Shirasawa, S (2004) Deregulated expression of KRAP, a novel gene encoding actin-interacting protein, in human colon cancer cells. J. Hum. Genet. 49:46-52 |
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| Abstract |
We have identified a novel gene, designated KRAP (Ki- ras-induced actin-interacting protein), encoding a protein of 1,259 amino acids with coiled-coil regions and transmembrane regions, from the cDNA library of human colon cancer HCT116 cells, as one of the genes upregulated by activated Ki- ras. While KRAP was rarely expressed in normal colon epithelium, deregulated constitutive KRAP expression was observed in some other colon cancer cells. In normal tissues, KRAP was strongly expressed in pancreas and testis. Anti-KRAP polyclonal antibodies detected endogenous KRAP as the molecular size of Mr 180,000, and immunofluorescence microscopy and cytochalasin E treatment revealed that KRAP was clearly associated with the actin filaments. Furthermore, KRAP was localized as a membrane-bound form with extracellular regions. These results together suggested KRAP might be involved in the regulation of filamentous actin and signals from the outside of the cells. |
| Links |
PubMed Online version:10.1007/s10038-003-0106-3 |
| Keywords |
Amino Acid Sequence; Biotinylation; Blotting, Northern; Cytochalasins; Fluorescent Antibody Technique; Gene Expression Regulation; Gene Library; Genes, ras/genetics; HCT116 Cells; Humans; Male; Membrane Proteins/genetics; Membrane Proteins/metabolism; Microfilament Proteins/genetics; Microfilament Proteins/metabolism; Molecular Sequence Data; Pancreas/metabolism; Precipitin Tests; Sequence Alignment; Sequence Analysis, DNA; Signal Transduction; Testis/metabolism; Transfection |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0051015: actin filament binding |
ECO:0000314: |
F |
Figure 4: co-localization of KRAP (homo sapien) with actin filaments using florescence antibodies. |
complete | ||||
|
enables |
GO:0051015: actin filament binding |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
See also
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