User:Watzadon

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
requireschanges	HALRU:ELYS	2012-04-15 13:43:03 CDT	GO:0009566 fertilization (P)	PMID:2377618	ECO:0000314 direct assay evidence used in manual assertion	Figure 1 shows the The species specificity of red and pink abalone lysins to dissolve isolated 125I-labeled VE, demonstrating the lysin activity of these proteins	challenge
requireschanges	APLCA:NADA	2012-04-15 13:56:05 CDT	GO:0003953 NAD+ nucleosidase activity (F)	PMID:1650254	ECO:0000314 direct assay evidence used in manual assertion	Figure 2 shows the direct assay of the extracted and purified protein and its NADase activity when nicotinamide is present. Figure 3 shows the enzymatic kinetics of this enzyme and its product formation.	challenge
requireschanges	HALRO:SPRM	2012-04-15 14:12:04 CDT	GO:0008233 peptidase activity (F)	PMID:6365918	ECO:0000314 direct assay evidence used in manual assertion	This paper alongside Figure 2 and Figure 3 describe the stability and ability of this enzyme to cleave the peptide sequence Val-Pro-Arg	challenge
requireschanges	RABIT:SP17	2012-04-15 14:36:56 CDT	GO:0007339 binding of sperm to zona pellucida (P)	PMID:7525387	ECO:0000314 direct assay evidence used in manual assertion	Figure 10 shows the binding of the sperm sp17 protein to the to zona pellucida region of egg cells	challenge
requireschanges	CAEEL:SPE9	2012-04-15 14:53:36 CDT	GO:0009566 fertilization (P)	PMID:9546393	ECO:0000315 mutant phenotype evidence used in manual assertion	This paper describes mutant SPE-9 deficient nematode spermatozoa that were unable to fertilize normal egg cells preventing egg fertilization. Figure 1 shows a graphical representation of the infertility and ovulation of SPE-9 mutant nematodes. Figure 2 and Figure 3 show microscope images of mutant spermatozoa cells	challenge
requireschanges	MOUSE:IZUM1	2012-04-15 15:00:43 CDT	GO:0007342 fusion of sperm to egg plasma membrane (P)	PMID:15759005	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 3 shows male infertility when Izumo gene is disrupted with insertional mutagensis	challenge

acceptable:0
unacceptable:0
requires_changes:6
flagged:0

Annotations challenged by Watzadon

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

0 annotations fixed by Watzadon

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	HUMAN:SSFA2	2012-03-27 11:54:38 CDT	GO:0051015 actin filament binding (F)	PMID:14673706	ECO:0000314 direct assay evidence used in manual assertion	Figure 4: co-localization of KRAP (homo sapien) with actin filaments using florescence antibodies.	challenge
unacceptable	?	2012-04-01 16:00:30 CDT	GO:0004474 (Figure 4 and Figure 5 show the effects of varying glyoxylate(A) and succinate (B) on the isocitrate lyase condensation reaction of the D475N mutant of the Euglena gracilis glyoxylate cycle enzyme)	malate synthase activity	IDA: Inferred from Direct Assay		challenge

acceptable:1
unacceptable:1
requires_changes:0
flagged:0

Annotations challenged by Watzadon

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

0 annotations fixed by Watzadon