Category:Team King of All Cosmos

Table 2: Strain AJ34 carrying the algF::ΩTc insertion in the non-polar orientation produced unacetylated alginate. Strain AJ21 carrying the algF::ΩTc mutation in the polar orientation did not produce alginate. Plasmid pRSA55 carrying the A. vinelandii algL-A region was transferred by conjugation to strain AJ34. This plasmid restored wild-type mucoid phenotype and alginate acetylation to AJ34 mutant.

Fig. 6A: While the vanillate conversion rate of the cell extract of DKLM incubated with vanillate was strikingly reduced, DDAM (ligM and desA double mutant) cells no longer showed any such activity. These results indicated that only ligM and desA are involved in vanillate O demethylation.

Fig. 6A: The vanillate conversion rate of the cell extract of DKLM (ligM mutant) incubated with vanillate was strikingly reduced. ligM plays a major role in the O demethylation of vanillate.

(Fig. 1A: The LPS from the algC mutants, PAO1 algC::tet and PAClR algC::tet did not produce the wildtype laddering pattern of smooth LPS. The laddering is because of O-antigen.)

Fig 1B: CONFIRMATION THAT LADDERING IS DUE TO EXPRESSION OF O-ANTIGENIC LPS: Polyclonal rabbit antisera raised to purified P. aeruginosa serotype 05 or serotype 03 LPS did not react to LPS recovered from the algC::tet mutant strains, while strong reactions occurred to LPS from the parental and complemented strains.

Figure 6C shows that the gene-product of the cloned genes they are studying absorbs UV-light, which is evidence that the gene-product is the honey bee UV-opsin.

Fig. 3: MUTANT PHENOTYPE: Mutations in

fimS result in either complete loss or severe retardation of twitching motility.

Fig 4: ELISA ASSAY: fimS mutant

essentially lacks surface fimbriae as shown by ELISA, which is a sensitive quantitative assay.

Figure 3E shows in detail that Rh5 protein is expressed in the rhabdomere of the fruit fly eye.

Figure 1C (small lower right box), shows the absorbance spectra of the gene product, when bound to the chromophore 11-cis retinal, before and after photoactivation with light.

Figure 1B, shows that the gene-product, when bound to 11-cis retinal, absorbs light, which induces a change to all trans-retinal and the resulting absorbance spectrum of the protein.

Figure 4, shows the absorbance spectrum of the gene product and the spectra of common mutants that affect the protein's light absorbing capabilities.

Figure 3, shows the absorbance spectra of the gene product and the resulting spectra that occur from mutations in the gene.

Figure 5 and Table 2 show that the gene-product of proA gene the group cloned and sequenced is of the same size and activity as the 4-hydroxy-2-oxoglutarate aldolase enzyme that was characterized in previous papers.

Fig 5: Confirms that rhy-1 inhibits the expression of HIF-1

target genes in a real-time RT-PCR assay monitoring the expression levels of two endogenous mRNAs, K10H10.2 and F22B5.4. Mutation of rhy-1 significantly increased the expression of both genes.

Fig 5 indicates that mutations in the secretion of EsxB cause a 2- to

4-log reduction in the ability of these mutant staphylococci to establish abscesses in infected mice--EsxA and EsxB represent an important virulence strategy in S. aureus.