PMID:9502802

Citation	Townson, SM, Chang, BS, Salcedo, E, Chadwell, LV, Pierce, NE and Britt, SG (1998) Honeybee blue- and ultraviolet-sensitive opsins: cloning, heterologous expression in Drosophila, and physiological characterization. J. Neurosci. 18:2412-22
Abstract	The honeybee (Apis mellifera) visual system contains three classes of retinal photoreceptor cells that are maximally sensitive to light at 440 nm (blue), 350 nm (ultraviolet), and 540 nm (green). We performed a PCR-based screen to identify the genes encoding the Apis blue- and ultraviolet (UV)-sensitive opsins. We obtained cDNAs that encode proteins having a high degree of sequence and structural similarity to other invertebrate and vertebrate visual pigments. The Apis blue opsin cDNA encodes a protein of 377 amino acids that is most closely related to other invertebrate visual pigments that are thought to be blue-sensitive. The UV opsin cDNA encodes a protein of 371 amino acids that is most closely related to the UV-sensitive Drosophila Rh3 and Rh4 opsins. To test whether these novel Apis opsin genes encode functional visual pigments and to determine their spectral properties, we expressed them in the R1-6 photoreceptor cells of blind ninaE mutant Drosophila, which lack the major opsin of the fly compound eye. We found that the expression of either the Apis blue- or UV-sensitive opsin in transgenic flies rescued the visual defect of ninaE mutants, indicating that both genes encode functional visual pigments. Spectral sensitivity measurements of these flies demonstrated that the blue and UV visual pigments are maximally sensitive to light at 439 and 353 nm, respectively. These maxima are in excellent agreement with those determined previously by single-cell recordings from Apis photoreceptor cells and provide definitive evidence that the genes described here encode visual pigments having blue and UV sensitivity.
Links	PubMed
Keywords	Animals; Base Sequence; Bees/genetics; Cloning, Molecular; Color; Drosophila/genetics; Gene Expression; Genes, Insect; Molecular Sequence Data; Rod Opsins/genetics; Sequence Homology, Amino Acid; Ultraviolet Rays
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
APIME:OPSB	involved_in	GO:0050908: detection of light stimulus involved in visual perception	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
APIME:OPSB		GO:0050908: visual perception	ECO:0000314:		P		Figure 5 & 6	complete CACAO 3516
APIME:OPSUV		GO:0009881: photoreceptor activity	ECO:0000314:		F		Figure 6C shows that the gene-product of the cloned genes they are studying absorbs UV-light, which is evidence that the gene-product is the honey bee UV-opsin.	complete CACAO 4319
APIME:OPSUV	enables	GO:0009881: photoreceptor activity	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete

References

See Help:References for how to manage references in GONUTS.