GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

Category:Team G-Nomes

From GONUTS
Jump to: navigation, search

SUPER BOWL ROUND Spring 2012

StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
requireschangesTHLCA:Q70LF4Pemmara1, Team G-Nomes2012-04-14 21:38:07 CDTGO:0046873 metal ion transmembrane transporter activity (F)PMID:21621506ECO:0000314 direct assay evidence used in manual assertion

Fig 2. shows that the ATPase activity of the transporter is activated by Cadmium, copper, and zinc

challenge
acceptableARATH:PEN1Pemmara1, Team G-Nomes2012-04-14 21:29:09 CDTGO:0016104 triterpenoid biosynthetic process (P)PMID:17474751ECO:0000314 direct assay evidence used in manual assertion

Fig 1. shows chromatograph results confirming the products of this enzyme

challenge
requireschangesCAPCH:O82520Pemmara1, Team G-Nomes2012-04-14 20:13:59 CDTGO:0004315 3-oxoacyl-(acyl-carrier-protein) synthase activity (F)PMID:12810854ECO:0000315 mutant phenotype evidence used in manual assertion

Table 2. recombinant enzyme increased the formation of long chain fatty acids

challenge
requireschanges9BURK:C3UVB8Pemmara1, Team G-Nomes2012-04-14 19:56:42 CDTGO:0051213 dioxygenase activity (F)PMID:19251893ECO:0000314 direct assay evidence used in manual assertion

Fig 4. shows how DPA in enzyme forms stoichiometric amounts of the expected products.

Fig 5. shows that the enzyme inserts both atoms of molecular oxygen into DPA, proving that it has dioxygenase activity

challenge
requireschangesRAT:MCL1Yujulian, Team G-Nomes2012-04-14 03:04:44 CDTGO:0043066 negative regulation of apoptotic process (P)PMID:21977009ECO:0000315 mutant phenotype evidence used in manual assertion

Fig.2a Mcl-1 protein expression by gradually decreases after PIC transfection

fig.2d Mcl-1 KO by siRNAs increase cleavage of caspase 3 and 9

challenge
requireschangesECOLI:UVRCYujulian, Team G-Nomes2012-04-14 02:51:05 CDTGO:0006289 nucleotide-excision repair (P)PMID:1387639ECO:0000315 mutant phenotype evidence used in manual assertion

Fig2, show mutations in His,Asp,Glu residues in catalytic domain affects ability to make a 5' incision

challenge
acceptableHUMAN:SCTM1Yujulian, Team G-Nomes2012-04-14 01:20:15 CDTGO:0016021 integral to membrane (C)PMID:9480746ECO:0000314 direct assay evidence used in manual assertion

Fig.3C western blot detected K12 protein in the insoluble pellet fraction after treatment with sodium carbonate(pH 11.5)

challenge
updatedbyinstructorHUMAN:RAG1Yujulian, Team G-Nomes2012-04-12 15:24:41 CDTGO:0033151 V(D)J recombination (P)PMID:14670978ECO:0000315 mutant phenotype evidence used in manual assertion

Fig.1 Mutation in amino acid residues of RAG1 decrease the cleavage activity and formation of nicks and hairpin structures

challenge
acceptableMYCTU:OTSAYujulian, Team G-Nomes2012-04-11 16:25:59 CDTGO:0005992 trehalose biosynthetic process (P)PMID:12473104ECO:0000314 direct assay evidence used in manual assertion

Fig.4 synthesis of trehalose using UDP-Glc and GDP-Glc correlates with amount or enzyme and has a linear relationship with time

Fig.5 increased trehalose-P synthesized correlates with increase amount of TPS protein.

challenge
requireschangesMUSAC:ACCOPemmara1, Team G-Nomes2012-04-10 11:17:58 CDTGO:0009693 ethylene biosynthetic process (P)PMID:10594112ECO:0000314 direct assay evidence used in manual assertion

Fig 4 shows how increased ethylene production goes hand in hand with increased enzyme activity

challenge
requireschangesKLEPN:ILVBPemmara1, Team G-Nomes2012-04-10 10:51:54 CDTGO:0003984 acetolactate synthase activity (F)PMID:1644303ECO:0000314 direct assay evidence used in manual assertion

Fig. 4 shows an assay of the protein purified from a heterlogous host

challenge


MSU Internal Competition

StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
requireschangesECOLI:ARGAYujulian, Team G-Nomes2012-03-27 12:34:59 CDTGO:0004358 glutamate N-acetyltransferase activity (F)PMID:9572954ECO:0000315 mutant phenotype evidence used in manual assertion

Fig.3 synthesis of arginine by expressing argA in plasmids

challenge
requireschanges9GAMM:C8YX87Pemmara1, Team G-Nomes2012-03-27 11:59:31 CDTGO:0004300 enoyl-CoA hydratase activity (F)PMID:19807777ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 3 shows that E. coli transformed with acu genes shows much higher 3-HP production. This supports this enzymes involvement in converting acrylate to 3-HP

challenge
updatedbyinstructorECOLI:ZAPCPemmara1, Team G-Nomes2012-03-27 11:52:28 CDTGO:0036212 contractile ring maintenance (P)PMID:21216995ECO:0000314 direct assay evidence used in manual assertion

Table 2, Fig 2 show that cells lacking or overexpressing ZapC have abnormal FtsZ ring morphologies

challenge
requireschanges9GAMM:C8YX88Pemmara1, Team G-Nomes2012-03-27 11:51:32 CDTGO:0008410 CoA-transferase activity (F)PMID:19807777ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 3 shows that E. coli transformed with acu genes shows much higher levels of 3-HP

challenge

Pages in category "Team G-Nomes"

The following 2 pages are in this category, out of 2 total.


Jump to pages starting with: P Y