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User:Pemmara1

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SUPER BOWL (Round 5)

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
requireschangesKLEPN:ILVB2012-04-10 10:51:54 CDTGO:0003984 acetolactate synthase activity (F)PMID:1644303ECO:0000314 direct assay evidence used in manual assertion

Fig. 4 shows an assay of the protein purified from a heterlogous host

challenge
requireschangesMUSAC:ACCO2012-04-10 11:17:58 CDTGO:0009693 ethylene biosynthetic process (P)PMID:10594112ECO:0000314 direct assay evidence used in manual assertion

Fig 4 shows how increased ethylene production goes hand in hand with increased enzyme activity

challenge
requireschanges9BURK:C3UVB82012-04-14 19:56:42 CDTGO:0051213 dioxygenase activity (F)PMID:19251893ECO:0000314 direct assay evidence used in manual assertion

Fig 4. shows how DPA in enzyme forms stoichiometric amounts of the expected products.

Fig 5. shows that the enzyme inserts both atoms of molecular oxygen into DPA, proving that it has dioxygenase activity

challenge
requireschangesCAPCH:O825202012-04-14 20:13:59 CDTGO:0004315 3-oxoacyl-(acyl-carrier-protein) synthase activity (F)PMID:12810854ECO:0000315 mutant phenotype evidence used in manual assertion

Table 2. recombinant enzyme increased the formation of long chain fatty acids

challenge
acceptableARATH:PEN12012-04-14 21:29:09 CDTGO:0016104 triterpenoid biosynthetic process (P)PMID:17474751ECO:0000314 direct assay evidence used in manual assertion

Fig 1. shows chromatograph results confirming the products of this enzyme

challenge
requireschangesTHLCA:Q70LF42012-04-14 21:38:07 CDTGO:0046873 metal ion transmembrane transporter activity (F)PMID:21621506ECO:0000314 direct assay evidence used in manual assertion

Fig 2. shows that the ATPase activity of the transporter is activated by Cadmium, copper, and zinc

challenge

acceptable:1
unacceptable:0
requires_changes:5
flagged:0

Annotations challenged by Pemmara1

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history

0 annotations fixed by Pemmara1


MSU Internal

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
updatedbyinstructorECOLI:ZAPC2012-03-27 11:52:28 CDTGO:0036212 contractile ring maintenance (P)PMID:21216995ECO:0000314 direct assay evidence used in manual assertion

Table 2, Fig 2 show that cells lacking or overexpressing ZapC have abnormal FtsZ ring morphologies

challenge
requireschanges9GAMM:C8YX882012-03-27 11:51:32 CDTGO:0008410 CoA-transferase activity (F)PMID:19807777ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 3 shows that E. coli transformed with acu genes shows much higher levels of 3-HP

challenge
requireschanges9GAMM:C8YX872012-03-27 11:59:31 CDTGO:0004300 enoyl-CoA hydratase activity (F)PMID:19807777ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 3 shows that E. coli transformed with acu genes shows much higher 3-HP production. This supports this enzymes involvement in converting acrylate to 3-HP

challenge

acceptable:0
unacceptable:0
requires_changes:2
flagged:0

Annotations challenged by Pemmara1

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history

0 annotations fixed by Pemmara1