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PMID:1644303
| Citation |
Peng, HL, Wang, PY, Wu, CM, Hwang, DC and Chang, HY (1992) Cloning, sequencing and heterologous expression of a Klebsiella pneumoniae gene encoding an FAD-independent acetolactate synthase. Gene 117:125-30 |
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| Abstract |
The gene encoding the valine-resistant and FAD-independent acetolactate synthase of Klebsiella pneumoniae was isolated and expressed in Escherichia coli. The nucleotide sequence of this gene was determined and it exhibited an open reading frame of 1680 bp in length. In vivo expression of the acetolactate synthase-encoding gene in E. coli revealed a single 60-kDa protein which is consistent with the molecular weight calculated from the deduced amino acid sequence of the gene product. The gene product shares about 20-30% homology with the acetolactate synthases of E. coli, yeast and higher plants. |
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| Keywords |
Acetolactate Synthase/genetics; Acetolactate Synthase/metabolism; Amino Acid Sequence; Base Sequence; Cloning, Molecular; Cosmids; DNA, Bacterial; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Flavin-Adenine Dinucleotide/metabolism; Klebsiella pneumoniae/enzymology; Klebsiella pneumoniae/genetics; Molecular Sequence Data; Plasmids; Pyruvate Decarboxylase/genetics; Pyruvate Decarboxylase/metabolism; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Sequence Homology, Nucleic Acid |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0003984: acetolactate synthase activity |
ECO:0000314: |
F |
Fig. 4 shows an assay of the protein purified from a heterlogous host |
complete | ||||
See also
References
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