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User:Yujulian

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SUPER BOWL (Round 5)

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableMYCTU:OTSA2012-04-11 16:25:59 CDTGO:0005992 trehalose biosynthetic process (P)PMID:12473104ECO:0000314 direct assay evidence used in manual assertion

Fig.4 synthesis of trehalose using UDP-Glc and GDP-Glc correlates with amount or enzyme and has a linear relationship with time

Fig.5 increased trehalose-P synthesized correlates with increase amount of TPS protein.

challenge
updatedbyinstructorHUMAN:RAG12012-04-12 15:24:41 CDTGO:0033151 V(D)J recombination (P)PMID:14670978ECO:0000315 mutant phenotype evidence used in manual assertion

Fig.1 Mutation in amino acid residues of RAG1 decrease the cleavage activity and formation of nicks and hairpin structures

challenge
acceptableHUMAN:SCTM12012-04-14 01:20:15 CDTGO:0016021 integral to membrane (C)PMID:9480746ECO:0000314 direct assay evidence used in manual assertion

Fig.3C western blot detected K12 protein in the insoluble pellet fraction after treatment with sodium carbonate(pH 11.5)

challenge
requireschangesECOLI:UVRC2012-04-14 02:51:05 CDTGO:0006289 nucleotide-excision repair (P)PMID:1387639ECO:0000315 mutant phenotype evidence used in manual assertion

Fig2, show mutations in His,Asp,Glu residues in catalytic domain affects ability to make a 5' incision

challenge
requireschangesRAT:MCL12012-04-14 03:04:44 CDTGO:0043066 negative regulation of apoptotic process (P)PMID:21977009ECO:0000315 mutant phenotype evidence used in manual assertion

Fig.2a Mcl-1 protein expression by gradually decreases after PIC transfection

fig.2d Mcl-1 KO by siRNAs increase cleavage of caspase 3 and 9

challenge

acceptable:2
unacceptable:0
requires_changes:2
flagged:0

Annotations challenged by Yujulian

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
requireschangesHowar123,
Team Militant Sharks
ALOAR:Q3L7F5GO:0030639 - polyketide biosynthetic process (P)PMID:16144421ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1

0 annotations fixed by Yujulian


MSU Internal

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
requireschangesECOLI:ARGA2012-03-27 12:34:59 CDTGO:0004358 glutamate N-acetyltransferase activity (F)PMID:9572954ECO:0000315 mutant phenotype evidence used in manual assertion

Fig.3 synthesis of arginine by expressing argA in plasmids

challenge

acceptable:0
unacceptable:0
requires_changes:1
flagged:0

Annotations challenged by Yujulian

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
requireschangesHowar123,
Team Militant Sharks
ALOAR:Q3L7F5GO:0030639 - polyketide biosynthetic process (P)PMID:16144421ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1

0 annotations fixed by Yujulian