Category:Team Biohazards

Figure 6.

Figure 6.

Figure 6C

Figure 6C

Figure 6C

Figure 6C

Figure 6C

Fig 3.

Supplemental Fig. S4A - The LIPL-4∷TagRFP protein fusion is observed in cytoplasmic bodies resembling autophagic granules.

Figure 2(A). LIPL-4 activates autophagy.

Figure 2. TrwK ATP hydrolase activity was monitored by the decrease of NADH absorbance at 340 nm

TrwK does not behave as an integral membrane protein(Paragraph) + Table 2

Researchers found that the addition of detergent and phospholipids didn't affect the solubility of TrwK in solution from which they infered that unlike homologs, TrwK was not an integral membrane protein.

Fig. 6 - ATP hydrolysis assay measured ATP concentration via ATPase assay.

Wild type TrwK showed a conjugation frequencies 4x10^-1 where a deletion in TrwK showed a conjugation frequency of 4x10^-6. This value is closer to the negative control of 4x10^-7 showing that TrwK is needed for conjugation.

As shown in figure 1C, wild type FtsK shows significant DNA-dependent ATPase activity in comparison to FtsK containing a mutation in the walker A motif, which sees significantly inhibited activity.

Fig. 2 - ATPase activity was monitored by decrease of NADH absorbance at 340 nm.

Fig. 3 - Kinetic analysis of ATPase activity

Figure 2. ConE is seen as being required for unidirectional conjugation between Bacillus subtilis bacterial cells, as cells deleted for conE or containing mutated conE fail to mate.

Figure 3. ConE bound to green fluorescent protein is seen localizing to the cell membrane, preferentially at the cell poles.