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PMID:23408623
| Citation |
Henaff, D, Rémillard-Labrosse, G, Loret, S and Lippé, R' (2013) Analysis of the early steps of herpes simplex virus type 1 capsid tegumentation. J. Virol. ' |
|---|---|
| Abstract |
Herpes simplex virus type 1 particles are multilayered structures with a DNA genome surrounded by a capsid, tegument and an envelope. While the protein content of mature virions is known, the sequence of addition of the tegument and the intracellular compartments where this occurs are intensely debated. To probe this process during the initial stages of egress, we used two approaches; an in vitro nuclear egress assay which reconstitutes the exit of nuclear capsids to the cytoplasm and a classical nuclear capsid sedimentation assay. As anticipated, in vitro cytoplasmic capsids did not harbor U(L)34, U(L)31 or viral glycoproteins but contained U(S)3. In agreement with previous findings, both nuclear and in vitro capsids were positive for ICP0 and ICP4. Unexpectedly, nuclear C-capsids and cytoplasmic capsids produced in vitro without any cytosolic viral proteins also scored positive for U(L)36 and U(L)37. Immuno-electron microscopy confirmed these tegument proteins were closely associated with nuclear capsids. When cytosolic viral proteins were present in the in vitro assay, no additional tegument proteins were detected on the capsids. As previously reported, the tegument was sensitive to high salt extraction but was surprisingly stabilized by exogenous proteins. Finally some tegument proteins seemed partially lost during egress while others were possibly added at multiple steps or modified along the way. Overall, an emerging picture hints at the early coating of capsids with up to 5 tegument proteins at the nuclear stage, the shedding of some viral proteins during nuclear egress, and the acquisition of others tegument proteins during re-envelopment. |
| Links |
PubMed Online version:10.1128/JVI.03292-12 |
| Keywords |
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| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0019028: viral capsid |
ECO:0000314: |
C |
Fig 3. |
complete | ||||
| GO:0019033: viral tegument |
ECO:0000314: |
C |
Figure 6C |
complete | ||||
|
part_of |
GO:0019033: viral tegument |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
| GO:0019033: viral tegument |
ECO:0000314: |
C |
Figure 6C |
complete | ||||
|
part_of |
GO:0019033: viral tegument |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0019033: viral tegument |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
| GO:0019033: viral tegument |
ECO:0000314: |
C |
Figure 6C |
complete | ||||
|
part_of |
GO:0019033: viral tegument |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
| GO:0019033: viral tegument |
ECO:0000314: |
C |
Figure 6C |
complete | ||||
|
part_of |
GO:0019033: viral tegument |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
| GO:0019033: viral tegument |
ECO:0000314: |
C |
Figure 6C |
complete | ||||
|
part_of |
GO:0019033: viral tegument |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
| GO:0019028: viral capsid |
ECO:0000314: |
C |
Figure 6. |
complete | ||||
|
part_of |
GO:0019028: viral capsid |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
| GO:0019028: viral capsid |
ECO:0000314: |
C |
Figure 6. |
complete | ||||
|
part_of |
GO:0019028: viral capsid |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
See also
References
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