User:Kjswerdlow

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	ECOLX:Q2NKG6	2013-03-04 08:30:28 CST	GO:0016887 ATPase activity (F)	PMID:18539740	ECO:0000314 direct assay evidence used in manual assertion	Fig. 2 - ATPase activity was monitored by decrease of NADH absorbance at 340 nm. Fig. 3 - Kinetic analysis of ATPase activity	challenge
acceptable	ECOLX:Q2NKG6	2013-03-04 08:50:45 CST	GO:0009291 unidirectional conjugation (P)	PMID:18539740	ECO:0000315 mutant phenotype evidence used in manual assertion	Wild type TrwK showed a conjugation frequencies 4x10^-1 where a deletion in TrwK showed a conjugation frequency of 4x10^-6. This value is closer to the negative control of 4x10^-7 showing that TrwK is needed for conjugation.	challenge
acceptable	9GAMM:A1YBN2	2013-03-04 09:00:40 CST	GO:0016887 ATPase activity (F)	PMID:20144229	ECO:0000314 direct assay evidence used in manual assertion	Fig. 6 - ATP hydrolysis assay measured ATP concentration via ATPase assay.	challenge
acceptable	ECOLX:Q2NKG6	2013-03-05 12:50:25 CST	GO:0016021 integral to membrane (C)	PMID:18539740	ECO:0000314 direct assay evidence used in manual assertion	TrwK does not behave as an integral membrane protein(Paragraph) + Table 2 Researchers found that the addition of detergent and phospholipids didn't affect the solubility of TrwK in solution from which they infered that unlike homologs, TrwK was not an integral membrane protein.	challenge

acceptable:4
unacceptable:0
requires_changes:0
flagged:0

Annotations challenged by Kjswerdlow

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

0 annotations fixed by Kjswerdlow

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