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ECOLX:Q2NKG6
Contents
| Species (Taxon ID) | Escherichia coli. (562) | |
| Gene Name(s) | trwK (ECO:0000313 with EMBL:FAA00030.1) | |
| Protein Name(s) | TrwK protein (ECO:0000313 with EMBL:FAA00030.1) | |
| External Links | ||
| UniProt | Q2NKG6 | |
| EMBL | BR000038 | |
| GO | GO:0005524 GO:0016887 GO:0006200 GO:0009291 | |
| Gene3D | 3.40.50.300 | |
| InterPro | IPR004346 IPR018145 IPR027417 | |
| Pfam | PF03135 | |
| SUPFAM | SSF52540 | |
| TIGRFAMs | TIGR00929 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
| GO:0016887 |
ATPase activity |
ECO:0000314 |
F |
Fig. 2 - ATPase activity was monitored by decrease of NADH absorbance at 340 nm. Fig. 3 - Kinetic analysis of ATPase activity |
complete | |||||
| GO:0009291 |
unidirectional conjugation |
ECO:0000315 |
P |
Wild type TrwK showed a conjugation frequencies 4x10^-1 where a deletion in TrwK showed a conjugation frequency of 4x10^-6. This value is closer to the negative control of 4x10^-7 showing that TrwK is needed for conjugation. |
complete | |||||
|
NOT |
GO:0016021 |
integral to membrane |
ECO:0000314 |
C |
TrwK does not behave as an integral membrane protein(Paragraph) + Table 2 Researchers found that the addition of detergent and phospholipids didn't affect the solubility of TrwK in solution from which they infered that unlike homologs, TrwK was not an integral membrane protein.
|
complete | ||||
|
enables |
GO:0016887 |
ATPase activity |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
NOT|part_of |
GO:0016021 |
integral component of membrane |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0009291 |
unidirectional conjugation |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0005524 |
ATP binding |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
