Category:Team BPS1420

Figure 5 (A) and 5 (B). yfh1 deletion clearly has resulted decrease number of Fe/S protein maturation in mitochondria compared to the wildtype.

Figure 4 (B) and 4(D). Dramatic lost of III2 and IV2 complex can be seen in the lanes of the rcf1 mutant when compared to the wildtype lanes.

Figure 2 (B). The lack of band in lane 14 and 16 compared to the WT bands at the specific high molecular weight of 230 and 440 kDa indicates the lack of complex IV in mutant cox4.

Figure 7A illusterates an increase in intracellular thiamine levels in the mutant Hst1 representing that cellular thiamine homeostasis in yeast requires Hst1.

Figure 4 (C). The blot of the gene ump1 mutant compared to the wild type blot, as long as the rest of the blots, indicate that the presence of ump1 is needed for regulation of core complex assembly due to the lack of regulatory particle and core particle bands.

Figure 5(A) The immunoblot analysis indicates that the blot of Ubp6 mutant accumulates ubiquitylated proteins unlike the wild type cells.

Figure 4 (D) Double deletion of Ubp6 and hsm3 shows the most noticeable growth defect due to lack of proteasomal assembly.