User:Turmoaik

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
updatedbyinstructor	YEAST:UBP6	2012-04-14 12:25:41 CDT	GO:0032434 regulation of proteasomal ubiquitin-dependent protein catabolic process (P)	PMID:21658604	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 5(A) The immunoblot analysis indicates that the blot of Ubp6 mutant accumulates ubiquitylated proteins unlike the wild type cells. Figure 4 (D) Double deletion of Ubp6 and hsm3 shows the most noticeable growth defect due to lack of proteasomal assembly.	challenge
updatedbyinstructor	YEAST:UMP1	2012-04-14 13:07:43 CDT	GO:0090363 regulation of proteasome core complex assembly (P)	PMID:21878652	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 4 (C). The blot of the gene ump1 mutant compared to the wild type blot, as long as the rest of the blots, indicate that the presence of ump1 is needed for regulation of core complex assembly due to the lack of regulatory particle and core particle bands.	challenge
updatedbyinstructor	YEAST:COX4	2012-04-15 13:36:35 CDT	GO:0033617 mitochondrial respiratory chain complex IV assembly (P)	PMID:22342701	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 2 (B). The lack of band in lane 14 and 16 compared to the WT bands at the specific high molecular weight of 230 and 440 kDa indicates the lack of complex IV in mutant cox4.	challenge
updatedbyinstructor	YEAST:RCF1	2012-04-15 13:47:20 CDT	GO:0097250 mitochondrial respiratory chain supercomplex assembly (P)	PMID:22342701	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 4 (B) and 4(D). Dramatic lost of III2 and IV2 complex can be seen in the lanes of the rcf1 mutant when compared to the wildtype lanes.	challenge
updatedbyinstructor	YEAST:FRDA	2012-04-15 14:09:31 CDT	GO:0016226 iron-sulfur cluster assembly (P)	PMID:12065597	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 5 (A) and 5 (B). yfh1 deletion clearly has resulted decrease number of Fe/S protein maturation in mitochondria compared to the wildtype.	challenge

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Annotations challenged by Turmoaik

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
requireschanges	Konalash, Team Celestial Seasonings	YERPE:Q74PX8	GO:0042710 - biofilm formation (P)	PMID:21559445	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 1

0 annotations fixed by Turmoaik

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
requireschanges	?	2012-04-01 11:28:28 CDT	GO:0004347 glucose-6-phosphate isomerase activity (Table 1. Demonstrates the importance of the gene PGI1 for phosphoglucose isomerase activity. The quantity of the reactive activity increases by 7 folds due to the addition of the plasmid including the PGI1 gene to yeast lacking this specific gene. )	PMID:3007940	IEP: Inferred from Expression Pattern		challenge

acceptable:0
unacceptable:0
requires_changes:1
flagged:0

Annotations challenged by Turmoaik

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
requireschanges	Konalash, Team Celestial Seasonings	YERPE:Q74PX8	GO:0042710 - biofilm formation (P)	PMID:21559445	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 1

0 annotations fixed by Turmoaik