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User:Konalash

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SUPER BOWL (Round 5)

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableCORGL:ILVC2012-04-12 23:29:45 CDTGO:0008677 2-dehydropantoate 2-reductase activity (F)PMID:12948643ECO:0000315 mutant phenotype evidence used in manual assertion

Table 2. Introduction of the ilvC expression vector significantly increases the ketopantoate reductase activity that is absent in the ilvc insertion mutant.

challenge
requireschangesPSEAE:LEP42012-04-14 20:04:27 CDTGO:0016485 protein processing (P)PMID:20338182ECO:0000315 mutant phenotype evidence used in manual assertion

supplementary figure 3: An increase in mass is observed for minor pilins FimU, PilV, PilW, PilX, and PilE when pilD is inactivated, which suggests they are processed by pilD.

challenge
acceptableCLOBH:A5I1L82012-04-14 21:35:27 CDTGO:0070417 cellular response to cold (P)PMID:21367479ECO:0000315 mutant phenotype evidence used in manual assertion

table 4. the growth rate of the cspB mutant is significantly lower than wild type at 15 and 20 degrees C.

challenge
requireschangesBURCE:O083502012-04-15 14:28:25 CDTGO:0033250 penicillinase activity (F)PMID:3046483ECO:0000316 genetic interaction evidence used in manual assertion

table 3. Penicillinase activity in a strain of E. coli with no background penicillinase activity was observed upon introduction of a vector containing penA from P. cepacia.

challenge
unacceptableVIBC3:A5F4R72012-04-15 15:12:07 CDTGO:0015628 protein secretion by the type II secretion system (P)PMID:9371445ECO:0000315 mutant phenotype evidence used in manual assertion

table 5. protease (secreted via the general secretory pathway/ type II secretion system) activity is significantly less in an epsC mutant compared to wild type.

challenge
requireschangesVIBC3:A5F4R52012-04-15 15:32:13 CDTGO:0043165 Gram-negative-bacterium-type cell outer membrane assembly (P)PMID:9371445ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 3b. In an epsE mutant, detection of proteins critical for proper outer membrane assembly are not observed as they are in the wild type strain.

challenge

acceptable:2
unacceptable:1
requires_changes:3
flagged:0

Annotations challenged by Konalash

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
requireschangesVacca005,
Team Dinosaurs are Great
VIBCH:TCPA2GO:0051701 - biological process involved in interaction with host (P)PMID:12055286ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 2

0 annotations fixed by Konalash


MSU Internal

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
requireschangesYERPE:Q74PX82012-03-29 16:20:17 CDTGO:0042710 biofilm formation (P)PMID:21559445ECO:0000315 mutant phenotype evidence used in manual assertion

fig. 3: a fully functional hmsT gene product is necessary for biofilm formation.

challenge
requireschangesVIBC3:A5F6K02012-04-01 15:58:29 CDTGO:0048870 cell motility (P)PMID:11260476ECO:0000315 mutant phenotype evidence used in manual assertion

fig. 2: fliA was expressed from an inducible promoter in S. typhimurium and V. cholerae fliA mutants and restored motility. (Code IGI also applies here).

fig. 3 fliA mutant has a truncated flagellum.

fig. 4. known sigma 28-dependent promoters have little/no activity in a fliA mutant. the promoters are upregulated upon introduction of a fliA overexpression vector.

challenge

acceptable:0
unacceptable:0
requires_changes:2
flagged:0

Annotations challenged by Konalash

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history

0 annotations fixed by Konalash