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PMID:12948643
| Citation |
Merkamm, M, Chassagnole, C, Lindley, ND and Guyonvarch, A (2003) Ketopantoate reductase activity is only encoded by ilvC in Corynebacterium glutamicum. J. Biotechnol. 104:253-60 |
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| Abstract |
Ketopantoate reductase catalyzes the second step of the pantothenate pathway after ketoisovalerate, common intermediate in valine, leucine and pantothenate biosynthesis. We show here that the Corynebacterium glutamicum ilvC gene is able to complement a ketopantoate reductase deficient Escherichia coli mutant. Thus ilvC, encoding acetohydroxyacid isomeroreductase, involved in the common pathway for branched-chained amino acids, also exhibits ketopantoate reductase activity. Enzymatic activity was confirmed by biochemical analysis in C. glutamicum. Furthermore, inactivation of ilvC in C. glutamicum leads to auxotrophy for pantothenate, indicating that ilvC is the only ketopantoate reductase- encoding gene in C. glutamicum. |
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| Keywords |
Alcohol Oxidoreductases/deficiency; Alcohol Oxidoreductases/genetics; Alcohol Oxidoreductases/metabolism; Amino Acids, Branched-Chain/biosynthesis; Amino Acids, Branched-Chain/genetics; Coenzymes/genetics; Coenzymes/metabolism; Corynebacterium/enzymology; Corynebacterium/genetics; Enzyme Activation; Escherichia coli/genetics; Escherichia coli/metabolism; Gene Expression Regulation, Bacterial/physiology; Gene Expression Regulation, Enzymologic/physiology; Genetic Complementation Test; Ketol-Acid Reductoisomerase; Recombinant Proteins/metabolism |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0008677: 2-dehydropantoate 2-reductase activity |
ECO:0000315: |
F |
Table 2. Introduction of the ilvC expression vector significantly increases the ketopantoate reductase activity that is absent in the ilvc insertion mutant. |
complete | ||||
|
enables |
GO:0008677: 2-dehydropantoate 2-reductase activity |
ECO:0000315: mutant phenotype evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
See also
References
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