PMID:12237316

Citation	Lerch-Gaggl, A, Haque, J, Li, J, Ning, G, Traktman, P and Duncan, SA (2002) Pescadillo is essential for nucleolar assembly, ribosome biogenesis, and mammalian cell proliferation. J. Biol. Chem. 277:45347-55
Abstract	Mutation of the zebrafish pescadillo gene blocks expansion of a number of tissues in the developing embryo, suggesting roles for its gene product in controlling cell proliferation. We report that levels of the pescadillo protein increase in rodent hepatocytes as they enter the cell cycle. Pescadillo protein localizes to distinct substructures of the interphase nucleus including nucleoli, the site of ribosome biogenesis. During mitosis pescadillo closely associates with the periphery of metaphase chromosomes and by late anaphase is associated with nucleolus-derived foci and prenucleolar bodies. Blastomeres in mouse embryos lacking pescadillo arrest at morula stages of development, the nucleoli fail to differentiate and accumulation of ribosomes is inhibited. We propose that in mammalian cells pescadillo is essential for ribosome biogenesis and nucleologenesis and that disruption to its function results in cell cycle arrest.
Links	PubMed Online version:10.1074/jbc.M208338200
Keywords	3T3 Cells; Animals; Cell Division/physiology; Cell Nucleolus/metabolism; Cell Nucleus/metabolism; Cells, Cultured; Embryo, Mammalian/physiology; Embryo, Mammalian/ultrastructure; Embryo, Nonmammalian; Female; Gene Targeting; Genotype; Liver/cytology; Liver/metabolism; Mice; Microscopy, Confocal; Nuclear Proteins/genetics; Nuclear Proteins/metabolism; Phenotype; Proteins/metabolism; Ribosomes/metabolism; Tissue Distribution; Zebrafish/genetics; Zebrafish/metabolism; Zebrafish Proteins
edit table

Annotations

Gene product	Qualifier	GO ID	GO term name	Evidence Code	with/from	Aspect	Notes	Status
edit table

References

See Help:References for how to manage references in GONUTS.