GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
|Status||Page||User||Date/Time||GO Term (Aspect)||Reference||Evidence||Notes||Links|
|ECOLI:YOEB||Lguthrie, Team Attenuators||2011-11-20 14:08:18 CST||GO:0008428 ribonuclease inhibitor activity (F)||PMID:19141481||ECO:0000269 experimental evidence used in manual assertion|
Figure 1 shows the down-regulation of RNase III activity by YmdB.
|HUMAN:TYRO||Jcooper, Team Attenuators||2011-10-23 12:35:52 CDT||GO:0042470 melanosome (C)||PMID:1145200||ECO:0000314 direct assay evidence used in manual assertion|
Fig. 5: normal melanosomes stain for Tyrosinase.
|HUMAN:AP3B1||Jcooper, Team Attenuators||2011-10-23 12:32:03 CDT||GO:0032438 melanosome organization (P)||PMID:11452004||ECO:0000315 mutant phenotype evidence used in manual assertion|
In Fig. 5, normal melanosomes stain for tyrosinase. HPS-2 (AP-3 beta mutant) melanosomes lack tyrosinase.
|CLOAB:Q97EQ3||Lguthrie, Team Attenuators||2011-10-23 10:00:36 CDT||GO:0045127 N-acetylglucosamine kinase activity (F)||PMID:21784936||ECO:0000314 direct assay evidence used in manual assertion|
Figure 3 shows that MurK is able phosphorylate the cell wall amino sugar GlcNAc and MurNAc of the C. acetobutylcum. Figure for shows the products of the reaction of MurK with amino sugars and Figure 5 shows the substrate specificity of MurK.
|ARATH:NRT21||Lguthrie, Team Attenuators||2011-10-22 20:51:25 CDT||GO:0015706 nitrate transport (P)||PMID:20561257||ECO:0000315 mutant phenotype evidence used in manual assertion|
Figure 1 shows that ATnar2.1 mutants do not growth on low nitrate in comparison to the wildtype. Additionally growth can be restored to a level that closely parallels WT growth rates by transformation with a myc-tagged AtNAR2.1).
Figure 2 shows that mutant lacking the ATNRT2.1 polypeptide exhibit poor growth on low nitrate (also see page 743).
|PSEAE:F5KE31||Lguthrie, Team Attenuators||2011-10-22 20:22:31 CDT||GO:0018860 anthranilate-CoA ligase activity (Figure 2 shows that the presence of PqsA, which functions as a ligase is necessary for CoA thioester formation. E.coli strains containing native PqsA demonstrate CoA ligase activity while control strains with a vector plasmid instead of PqsA do not. )||PMID:18083812||IMP: Inferred from Mutant Phenotype||challenge|
|ECOLI:USPA||Jcooper, Team Attenuators||2011-10-22 13:18:50 CDT||GO:0015968 stringent response (P)||PMID:18039766||ECO:0000270 expression pattern evidence used in manual assertion|
In paragraph 5 of section "MG1655 response to SHX treatment at 10 min.", uspA is reported to be up-regulated in the first 5 min. of stringent response from amino acid starvation. Data shown in Supp. Table 1.
|ECOLI:USPA||Jcooper, Team Attenuators||2011-10-22 12:47:50 CDT||GO:0006950 response to stress (P)||PMID:1453957||ECO:0000270 expression pattern evidence used in manual assertion|
Fig. 1B and Fig. 8 show uspA expression in response to growth inhibition. "Induction is related to growth inhibition" section, p.3188, rules out uspA response to specific macronutrient starvation.
|BACTN:Q8A2Y3||Lguthrie, Team Attenuators||2011-09-25 14:25:33 CDT||GO:0009758 carbohydrate utilization (P)||PMID:16735464||ECO:0000315 mutant phenotype evidence used in manual assertion|
Fig. 3. GeneChip analysis comparing differential gene expression during in the glycotic pathway in WT and BT3172 deficient mice demonstrated that the absence of BT3172 impairs glycolysis. BT3172 specifically affected alpha-mannosidase gene expression.
|RHOCA:Q9L906||Jcooper, Team Attenuators||2011-09-25 14:23:07 CDT||GO:0046777 protein autophosphorylation (P)||PMID:10347192||ECO:0000314 direct assay evidence used in manual assertion|
|RHOCA:Q9L906||Jcooper, Team Attenuators||2011-09-25 14:18:32 CDT||GO:0016301 kinase activity (F)||PMID:10347192||ECO:0000314 direct assay evidence used in manual assertion|
|RHOCA:Q9L906||Jcooper, Team Attenuators||2011-09-25 14:18:32 CDT||GO:0000160 two-component signal transduction system (phosphorelay) (P)||PMID:10347192||ECO:0000314 direct assay evidence used in manual assertion|
|RHOCB:D5AQX9||Jcooper, Team Attenuators||2011-09-25 14:04:34 CDT||GO:0000156 two-component response regulator activity (F)||PMID:10347192||ECO:0000314 direct assay evidence used in manual assertion|
Figure 5 shows DNA affinity
|CLOTH:A3DBH0||Lguthrie, Team Attenuators||2011-09-25 12:01:06 CDT||GO:0016987 sigma factor activity (F)||PMID:20937888||ECO:0000314 direct assay evidence used in manual assertion|
Fig. 4. In vitro runoff transcription assay with purified C. thermocellum RNA polymerase and linear DNA containing the putative σI1 promoter sequence resulted in transcription from the sigI1 and celS promoters.
|RHOCB:D5AQX9||Jcooper, Team Attenuators||2011-09-24 15:27:11 CDT||GO:0000160 two-component signal transduction system (phosphorelay) (P)||PMID:10347192||ECO:0000314 direct assay evidence used in manual assertion|
Figures 2, 3