User:Lguthrie

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	CLOTH:A3DBH0	2011-09-25 12:01:06 CDT	GO:0016987 sigma factor activity (F)	PMID:20937888	ECO:0000314 direct assay evidence used in manual assertion	Fig. 4. In vitro runoff transcription assay with purified C. thermocellum RNA polymerase and linear DNA containing the putative σI1 promoter sequence resulted in transcription from the sigI1 and celS promoters.	challenge
acceptable	BACTN:Q8A2Y3	2011-09-25 14:25:33 CDT	GO:0009758 carbohydrate utilization (P)	PMID:16735464	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig. 3. GeneChip analysis comparing differential gene expression during in the glycotic pathway in WT and BT3172 deficient mice demonstrated that the absence of BT3172 impairs glycolysis. BT3172 specifically affected alpha-mannosidase gene expression.	challenge
requireschanges	PSEAE:F5KE31	2011-10-22 20:22:31 CDT	GO:0018860 anthranilate-CoA ligase activity (Figure 2 shows that the presence of PqsA, which functions as a ligase is necessary for CoA thioester formation. E.coli strains containing native PqsA demonstrate CoA ligase activity while control strains with a vector plasmid instead of PqsA do not. )	PMID:18083812	IMP: Inferred from Mutant Phenotype		challenge
acceptable	ARATH:NRT21	2011-10-22 20:51:25 CDT	GO:0015706 nitrate transport (P)	PMID:20561257	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 1 shows that ATnar2.1 mutants do not growth on low nitrate in comparison to the wildtype. Additionally growth can be restored to a level that closely parallels WT growth rates by transformation with a myc-tagged AtNAR2.1). Figure 2 shows that mutant lacking the ATNRT2.1 polypeptide exhibit poor growth on low nitrate (also see page 743).	challenge
acceptable	CLOAB:Q97EQ3	2011-10-23 10:00:36 CDT	GO:0045127 N-acetylglucosamine kinase activity (F)	PMID:21784936	ECO:0000314 direct assay evidence used in manual assertion	Figure 3 shows that MurK is able phosphorylate the cell wall amino sugar GlcNAc and MurNAc of the C. acetobutylcum. Figure for shows the products of the reaction of MurK with amino sugars and Figure 5 shows the substrate specificity of MurK.	challenge
requireschanges	ECOLI:YOEB	2011-11-20 14:08:18 CST	GO:0008428 ribonuclease inhibitor activity (F)	PMID:19141481	ECO:0000269 experimental evidence used in manual assertion	Figure 1 shows the down-regulation of RNase III activity by YmdB.	challenge

acceptable:4
unacceptable:0
requires_changes:2
flagged:0

Annotations challenged by Lguthrie

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

0 annotations fixed by Lguthrie