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My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableCLOTH:A3DBH02011-09-25 12:01:06 CDTGO:0016987 sigma factor activity (F)PMID:20937888ECO:0000314 direct assay evidence used in manual assertion

Fig. 4. In vitro runoff transcription assay with purified C. thermocellum RNA polymerase and linear DNA containing the putative σI1 promoter sequence resulted in transcription from the sigI1 and celS promoters.

acceptableBACTN:Q8A2Y32011-09-25 14:25:33 CDTGO:0009758 carbohydrate utilization (P)PMID:16735464ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 3. GeneChip analysis comparing differential gene expression during in the glycotic pathway in WT and BT3172 deficient mice demonstrated that the absence of BT3172 impairs glycolysis. BT3172 specifically affected alpha-mannosidase gene expression.

requireschangesPSEAE:F5KE312011-10-22 20:22:31 CDTGO:0018860 anthranilate-CoA ligase activity (Figure 2 shows that the presence of PqsA, which functions as a ligase is necessary for CoA thioester formation. E.coli strains containing native PqsA demonstrate CoA ligase activity while control strains with a vector plasmid instead of PqsA do not. )PMID:18083812IMP: Inferred from Mutant Phenotype
acceptableARATH:NRT212011-10-22 20:51:25 CDTGO:0015706 nitrate transport (P)PMID:20561257ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1 shows that ATnar2.1 mutants do not growth on low nitrate in comparison to the wildtype. Additionally growth can be restored to a level that closely parallels WT growth rates by transformation with a myc-tagged AtNAR2.1).

Figure 2 shows that mutant lacking the ATNRT2.1 polypeptide exhibit poor growth on low nitrate (also see page 743).

acceptableCLOAB:Q97EQ32011-10-23 10:00:36 CDTGO:0045127 N-acetylglucosamine kinase activity (F)PMID:21784936ECO:0000314 direct assay evidence used in manual assertion

Figure 3 shows that MurK is able phosphorylate the cell wall amino sugar GlcNAc and MurNAc of the C. acetobutylcum. Figure for shows the products of the reaction of MurK with amino sugars and Figure 5 shows the substrate specificity of MurK.

requireschangesECOLI:YOEB2011-11-20 14:08:18 CSTGO:0008428 ribonuclease inhibitor activity (F)PMID:19141481ECO:0000269 experimental evidence used in manual assertion

Figure 1 shows the down-regulation of RNase III activity by YmdB.



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