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User:Puthuveetilnp

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Phage Hunters Spring 2016

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
unacceptable9CAUD:A0A0K2FL492016-04-11 10:13:54 CDTGO:0016987 sigma factor activity (F)GO_REF:0000100ECO:0000250 sequence similarity evidence used in manual assertion

On Blastp, gp34 for Bacillus phage SPO1 had a query cover of 43%, E-value of 1e-09, and an identity of 45% when blasted against Gene 182 in Eyuki. HHpred results were the similar for Bacillus phage SPO1 and Gene 182 in Eyuki. Phage SPO1 results were: probability of 99.9% and E-value of 1.4E-26. Gene 182 in Eyuki results were: probability of 99.9% and E-value of 6.3E-24. Both were matched to protein 1OR7, a RNA Polymerase Sigma-E factor.

challenge
unacceptableBPSP1:RP342016-04-11 10:34:48 CDTGO:0016987 sigma factor activity (F)PMID:6441846ECO:0000314 direct assay evidence used in manual assertion

Refer to Figure 2 which shows how the function of gene 34 on Bacillus SPO1 was determined. A vector plasmid was used to help identify function. A solution of isolated RNA polymerases were inserted into lane 5 in the gel causing an aggregation of proteins at specific locations.

challenge
unacceptableBPSP1:B6V2P52016-04-18 06:14:39 CDTGO:0098026 virus tail, tube (In this paper, specifically in the section about tail assembly proteins, the authors discuss the potential function of gene 10.1. They speculate it might be a tail tube protein because the length of gene 10.1 corresponds to a gene that codes for a tail tube protein. Additionally, figure 5 shows how the authors analyzed other homologs to show how 10.01 was a tail tube protein.)PMID:19285085IGC: Inferred from Genomic Context UniProtKB:B6V2P4
challenge
unacceptable9CAUD:A0A0K2D0342016-04-23 23:54:22 CDTGO:0098026 virus tail, tube (C)other:GO_REF:0000100ECO:0000247 sequence alignment evidence used in manual assertion

Blastp results shows that this gene TsarBomba_87 and SPO1 10.1 has a query cover of 97%, E-value of 7e-43, and identity of 46%.

challenge
unacceptable9CAUD:S5YD002016-04-23 23:54:58 CDTGO:0098026 virus tail, tube (C)other:GO_REF:0000100ECO:0000247 sequence alignment evidence used in manual assertion

Blastp results for gene 85 for Troll and gene 10.1 for SPO1 have a query cover of 97%, E-value of 6e-41, and identity of 44%.

challenge
unacceptable9CAUD:A0A076G7112016-04-23 23:55:06 CDTGO:0098026 virus tail, tube (Blastp results for gp_186 for Bobb and gene 10.1 for SPO1 are a query cover of 97%, E-value of 2e-41, and identity of 44%.)other:GO_REF:0000100ISA: Inferred from Sequence Alignment UniProtKB:A0A076G711

UniProtKB:B6V2P5

challenge
unacceptable9CAUD:G9J2132016-04-23 23:55:27 CDTGO:0098026 virus tail, tube (The blast results of gene BCP78_0213 with SP01 10.1 gave these results: query cover of 97%, E-value of 7e-43, and identity of 46%.)other:GO_REF:0000100ISA: Inferred from Sequence Alignment UniProtKB:B6V2P5

UniProtKB:G9J213

challenge
unacceptableBPSP1:B6V2P52016-04-27 09:38:50 CDTGO:0098026 virus tail, tube (In Figure 3, a protein gel was run on Bacillus phage SP01 tails. Two proteins were identified (look at the T section of the gel), T3 and T6. T6 is identified as a core protein and is said to be a very abundant small protein in tails. This implies that T6 is a tail core protein, which is otherwise known as a tail tube.)PMID:6827651IDA: Inferred from Direct Assay
challenge

acceptable:0
unacceptable:8
requires_changes:0
flagged:0

Annotations challenged by Puthuveetilnp

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
unacceptableMnguy1,
Team Blue B
9CAUD:A0A0K2D0H8GO:0006508 - proteolysis (P)PMID:23688818ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 4
unacceptableJheins2,
Team Green B
ECOLX:A0A023Z1P7GO:2000966 - regulation of cell wall polysaccharide catabolic process (P)PMID:16615077ECO:0000250 sequence similarity evidence used in manual assertionchallengeC: 2
unacceptableVumh,
Team Kendrick Jafars
LEGPH:COAEGO:0004140 - dephospho-CoA kinase activity (F)PMID:25449315ECO:0000314 direct assay evidence used in manual assertionchallengeC: 3
unacceptableNitika1,
Team Purple A
9CAUD:G9J205GO:0008933 - lytic transglycosylase activity (F)PMID:15150235ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1
unacceptableMnguy1,
Team Blue B
BACTU:A0A0G3E6U5GO:0016301 - kinase activity (BlastP sequence alignment showed an E value of 7E-54 )PMID:11292795ISS: Inferred from Sequence Similarity UniProtKB:A0A0G3E6U5 challengeC: 3
unacceptableSpanch1,
Team Red B
9CAUD:A0A0K2D0G3GO:0016032 - viral process (P)PMID:3488191ECO:0000250 sequence similarity evidence used in manual assertionchallengeC: 2
unacceptableJefferyPowell,
Team T1
BPHK7:Q9MCN1GO:1905138 - positive regulation of viral DNA genome packaging via site-specific sequence recognition (P)PMID:24711378ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1

0 annotations fixed by Puthuveetilnp

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