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User:Vumh

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Phage Hunters Spring 2016

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
unacceptableLEGPH:COAE2016-04-05 10:00:29 CDTGO:0004140 dephospho-CoA kinase activity (F)PMID:25449315ECO:0000314 direct assay evidence used in manual assertion

Dephosphocoenzyme A kinase (DPCK; CoaE) catalyses the phosphorylation of the 3′-hydroxyl group of the ribose moiety of dephospho-CoA.

challenge
unacceptableBPT4:TERL2016-04-11 10:41:11 CDTGO:0098009 virus terminase, large subunit (C)PMID:19109896ECO:0000314 direct assay evidence used in manual assertion

Fig 1 of the paper illustrates gp17 as a large terminase.

challenge
unacceptableECOLI:RBN2016-04-11 03:39:06 CDTGO:0004519 zinc ion binding (F)PMID:16452444ECO:0000314 direct assay evidence used in manual assertion

In Fig. 1b, the ZiPD dimerization interface is in the region of helices α1, α2, and α3, and the dimer is arranged with the monomers in a head-to-head fashion with the exosite tails extending away from each other. A narrow cleft is formed jointly by the two monomers in which the active site and metal coordination residues are present.

challenge
unacceptableAQUAE:DNAG2016-04-22 20:33:44 CDTGO:0003896 DNA primase activity (F)PMID:16285921ECO:0000314 direct assay evidence used in manual assertion

In figure 1a, The conserved b-sheet surface of the ZBD contacts the RPD, associating via several salt bridges and polar interactions, but is

angled away by 45º to form a narrow groove between the two domains.

challenge
unacceptable9CAUD:A0A0K2FLA22016-05-16 20:29:13 CDTGO:0003896 DNA primase activity (F)other:GO:0000100ECO:0000255 match to sequence model evidence used in manual assertion

With an HHPred result of 100% with an e-value of 3.7e-46, the protein expressed in Eyuki130 is homologous to the one expressed in the VF5 strain of A. aeolicus.

challenge
unacceptable9CAUD:A0A0K2D0G32016-05-16 20:48:33 CDTGO:0098009 virus terminase, large subunit (C)other:GO:0000100ECO:0000247 sequence alignment evidence used in manual assertion

Blastp results show homology with Bacillus phage TsarBomba041 with a query coverage of 99% and 73% identity.

challenge

acceptable:0
unacceptable:6
requires_changes:0
flagged:0

What do the icons mean in the status column?

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