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Corn, JE, Pease, PJ, Hura, GL and Berger, JM (2005) Crosstalk between primase subunits can act to regulate primer synthesis in trans. Mol. Cell 20:391-401


The coordination of primase function within the replisome is an essential but poorly understood feature of lagging strand synthesis. By using crystallography and small-angle X-ray scattering (SAXS), we show that functional elements of bacterial primase transition between two dominant conformations: an extended form that uncouples a regulatory domain from its associated RNA polymerase core and a compact state that sequesters the regulatory region from the site of primer synthesis. FRET studies and priming assays reveal that the regulatory domain of one primase subunit productively associates with nucleic acid that is bound to the polymerase domain of a second protomer in trans. This intersubunit interaction allows primase to select initiation sites on template DNA and implicates the regulatory domain as a "molecular brake" that restricts primer length. Our data suggest that the replisome may cooperatively use multiple primases and this conformational switch to control initiation frequency, processivity, and ultimately, Okazaki fragment synthesis.


PubMed Online version:10.1016/j.molcel.2005.09.004


Bacteriophage T7/enzymology; Crystallography, X-Ray/methods; DNA/chemistry; DNA/metabolism; DNA Primase/chemistry; DNA Primase/metabolism; DNA Replication/physiology; Escherichia coli/enzymology; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/metabolism; Oligoribonucleotides/biosynthesis; Oligoribonucleotides/chemistry; Protein Structure, Tertiary; Viral Proteins/chemistry; Viral Proteins/metabolism



Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status


GO:0003896: DNA primase activity



In figure 1a, The conserved b-sheet surface of the ZBD contacts the RPD, associating via several salt bridges and polar interactions, but is angled away by 45º to form a narrow groove between the two domains.

CACAO 11887


See also


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