User:Mwwood2

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
updatedbyinstructor	ALIFS:LUXR	2013-09-29 15:45:16 CDT	GO:0045893 positive regulation of transcription, DNA-templated (P)	PMID:1987152	ECO:0000314 direct assay evidence used in manual assertion	Figure 2 shows the dual requirement for both IPTG and autoinducer to increase luciferase synthesis from pGS107 (lux R mutant strain) demonstrated that LuxR was able to activate transcription of operon lux in an autoinducer-dependent fashion.	challenge
updatedbyinstructor	ALIFS:LUXA	2013-09-29 16:26:31 CDT	GO:0008218 bioluminescence (P)	PMID:10913092	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 2. Juvenile squid exposed to strain ESR1 showed the typical induction of luminescence, which becomes apparent between 12 and 24 h postinfection. However, animals colonized by either theluxA (luciferase) or the luxR or luxI(regulatory) mutant failed to produce detectable levels of luminescence.	challenge
unacceptable	HUMAN:CH60	2013-10-13 13:51:37 CDT	GO:0006457 protein folding (P)	PMID:2528694	ECO:0000314 direct assay evidence used in manual assertion	As seen in figure 4a, addition of ATP is required for proper release and folding into protease resistant conformation of protein from hsp60	challenge
acceptable	ECOLI:CH10	2013-10-13 14:20:08 CDT	GO:0006457 protein folding (P)	PMID:2192863	ECO:0000314 direct assay evidence used in manual assertion	Figure 2a shows the effect of addition of GroES and ATP to a arrested GroEL protein folding of pre-beta-lactamase. Enzyme function dramatically increases after addition,suggesting GroES is required for proper protein folding	challenge
updatedbyinstructor	GEOMG:Q39Q67	2013-10-27 16:51:45 CDT	GO:0009013 succinate-semialdehyde dehydrogenase [NAD(P)+] activity (F)	PMID:22037946	ECO:0000314 direct assay evidence used in manual assertion	Figure 3 shows the succinic semialdehyde reducing ability to reducing SSA and demonstrated that NADPH/NADP+ is the suitable co-factor for these enzymes.	challenge
unacceptable	GEOMG:Q39Q67	2013-10-27 17:00:34 CDT	GO:0009013 succinate-semialdehyde dehydrogenase [NAD(P)+] activity (F)	PMID:22037946	ECO:0000247 sequence alignment evidence used in manual assertion	Table 4 shows the similarities in sequence between the SSAR proteins from Geobacter sulfurreducens and Geobacter metallireducens as compared to Arabidopsis thaliana, showing 64% sequence similarity	challenge
acceptable	MYCS2:PAT	2013-11-10 15:07:15 CST	GO:0008080 N-acetyltransferase activity (F)	PMID:21627103	ECO:0000314 direct assay evidence used in manual assertion	Figure 6 shows that this particular GMAT protein acts as a protein acetyltransferase and specifically acetylates the ε-amino group of a highly conserved lysine residue in acetyl-CoA synthetase . This acetylation results in the inactivation of ACS activity. Lysine acetylation by MsPat is dependent on 3',5'-cyclic adenosine monophosphate (cAMP), an important second messenger, indicating that MsPat is a downstream target of the intracellular cAMP signaling pathway	challenge
requireschanges	AZOBR:GLNB	2013-11-10 16:23:11 CST	GO:0006808 regulation of nitrogen utilization (P)	PMID:23144248	ECO:0000314 direct assay evidence used in manual assertion	Figure 1. Preincubation of the Fe protein with DraT in the presence of increasing concentrations of GlnB resulted in Fe protein inhibition in a GlnB-dependent manner	challenge

acceptable:2
unacceptable:2
requires_changes:1
flagged:0

Annotations challenged by Mwwood2

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
unacceptable	Sabrinamoisan, Team Go Pro	CHLRE:Q68RJ5	GO:0042384 - cilium assembly (P)	PMID:23990561	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 2
unacceptable	Meganmikkelson, Team Don't be anno-hating	HUMAN:AL1B1	GO:0004029 - aldehyde dehydrogenase (NAD+) activity (F)	PMID:401580	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 3
unacceptable	Meganmikkelson, Team Don't be anno-hating	SOLLC:F2YHL2	GO:0048102 - autophagic cell death (P)	PMID:21867679	ECO:0000316 genetic interaction evidence used in manual assertion	challenge	C: 1
requireschanges	Drl5141, Team Protein Titans	HUMAN:KSYK	GO:1901224 - positive regulation of non-canonical NF-kappaB signal transduction (P)	PMID:19151749	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 1
updatedbyinstructor	Tvelk, Team Pitbull Hurricanes	MAIZE:TIP44	GO:0015840 - urea transport (P)	PMID:22360887	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 2 fixed by Mwwood2

1 annotations fixed by Mwwood2

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