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Category:Team Go Pro

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StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableMOUSE:NLRC3Drl5141, Team Go Pro2013-11-10 17:28:19 CSTGO:0032720 negative regulation of tumor necrosis factor production (P)PMID:22863753ECO:0000315 mutant phenotype evidence used in manual assertion

With knockout mice, Figure 4c graph 1 shows that with stimulation of the TLR on the cell that there is an increased production of the TNF when compared to the WT.

challenge
acceptableMOUSE:NLRC3Drl5141, Team Go Pro2013-11-10 17:28:19 CSTGO:0032715 negative regulation of interleukin-6 production (P)PMID:22863753ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4c graph 2. In knockout mice of the NLCR3 protein then in response to stimulation of TLR then there is an increased amount of IL-6 made when compared to the WT cell.

challenge
acceptableMOUSE:NLRC3Drl5141, Team Go Pro2013-11-10 17:28:19 CSTGO:0032695 negative regulation of interleukin-12 production (P)PMID:22863753ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4c graph 4. With knockout mice of NLRC3 protein when TLR was stimulated there was an increased production of IL-12 when compared to the WT thus indicating that NLRC3 negatively regulates the IL-12 production.

challenge
acceptableMOUSE:NLRC3Drl5141, Team Go Pro2013-11-10 17:28:19 CSTGO:0032691 negative regulation of interleukin-1 beta production (P)PMID:22863753ECO:0000315 mutant phenotype evidence used in manual assertion

With knockdown of protein NLRC3 in mice, figure 4c graph 3 indicates that these mice upon TLR stimulation resulted in increased production of IL-1 beta when compared to the WT mice TLR response. This indicates that NLRC3 negatively regulates the production if IL-1 beta.

challenge
acceptableHUMAN:NLRC3Drl5141, Team Go Pro2013-11-10 17:02:27 CSTGO:0032715 negative regulation of interleukin-6 production (P)PMID:22863753ECO:0000314 direct assay evidence used in manual assertion

Figure 1d the second graph indicate that with overexpressed NLRC3 this decreases production of IL-6 in HEK293 t cells.

challenge
acceptableHUMAN:NLRC3Drl5141, Team Go Pro2013-11-10 17:02:27 CSTGO:0032720 negative regulation of tumor necrosis factor production (P)PMID:22863753ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1c shows when NLRC3 is not expressed in HEK293 T cells then the production of TNF in these cells increases indicating that NLRC3 negatively regulates TNF production.

challenge
updatedbyinstructorHUMAN:NLRC3Drl5141, Team Go Pro2013-11-10 17:02:27 CSTGO:0032715 negative regulation of interleukin-6 production (P)PMID:22863753ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1c shows that when NLRC3 is not expressed concentrations of IL-6 increase indicating that NLRC3 negatively regulates IL-6 production in HEK293 T cells.

challenge
acceptableHUMAN:NLRC3Drl5141, Team Go Pro2013-11-10 17:02:26 CSTGO:0032720 negative regulation of tumor necrosis factor production (P)PMID:22863753ECO:0000314 direct assay evidence used in manual assertion

Overexpression of NLRC3 led to decreased production of TNF through the MyD88 pathway of Hek293 T cells as shown in figure 1b.

challenge
acceptableHUMAN:TRAIPDrl5141, Team Go Pro2013-11-10 16:21:34 CSTGO:0010804 negative regulation of tumor necrosis factor-mediated signaling pathway (P)PMID:19151749ECO:0000314 direct assay evidence used in manual assertion

Increasing amounts of TRIP results in decreased activation of NF-kB by the TNF mediated pathway. This is indicated in Figure 6 and Supplemental figure 3.

challenge
updatedbyinstructorHUMAN:KSYKDrl5141, Team Go Pro2013-11-10 16:08:42 CSTGO:0010803 regulation of tumor necrosis factor-mediated signaling pathway (P)PMID:19151749ECO:0000315 mutant phenotype evidence used in manual assertion

SKY expression led to increased activity of the the TNF signaling pathway of resulting in activation of NF-kB as shown in Figure 5 particularly in C and D. Thus sky positively regulates TNF mediated signaling pathway.

challenge
requireschangesHUMAN:KSYKDrl5141, Team Go Pro2013-11-10 15:48:38 CSTGO:0005737 cytoplasm (C)PMID:19151749ECO:0000314 direct assay evidence used in manual assertion

Figure 4c and D show that when SKY and TRIAP in Human breast cells MCF7 cells are coexpressed then both are found in the cytoplasm. This is not normal for SKY which is normally found throughout the cell.

challenge
requireschangesHUMAN:TRAIPDrl5141, Team Go Pro2013-11-10 15:36:03 CSTGO:0005737 cytoplasm (C)PMID:19151749ECO:0000314 direct assay evidence used in manual assertion

Figure 4c and D shows when Sky-EGFP and TRIP were coexpressed then TRIP is colocalized along with SKY in the cytoplasm.

challenge
requireschangesHUMAN:TRAIPDrl5141, Team Go Pro2013-11-10 15:18:06 CSTGO:0034504 protein localization to nucleus (P)PMID:19151749ECO:0000314 direct assay evidence used in manual assertion

figure 4A shows Trip colocalized into the nucleoli of the nucleus via immunoflorescence of MCF7 cells with overexpressed TRIP.

challenge
requireschangesHUMAN:TRAIPDrl5141, Team Go Pro2013-11-10 15:18:06 CSTGO:0034504 protein localization to nucleus (P)PMID:19151749ECO:0000315 mutant phenotype evidence used in manual assertion

Trip knockdown with siRNA in figure 4B leads to reduced TRIP in the nucleoli of the nucleus in MCF7 cells showing that when trip is being expressed it is localized in the nucleoli of the nucleus.

challenge
unacceptableBACIU:Q5Y5R6Sabrinamoisan, Team Go Pro2013-11-05 21:16:07 CSTGO:1900734 positive regulation of polyketide biosynthetic process (P)PMID:24187085ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 6

challenge
unacceptableBACIU:Q0PEX8Sabrinamoisan, Team Go Pro2013-11-05 20:59:17 CSTGO:1900732 regulation of polyketide biosynthetic process (P)PMID:24187085ECO:0000315 mutant phenotype evidence used in manual assertion

Figure S1

challenge
updatedbyinstructorPIG:TNFADrl5141, Team Go Pro2013-11-04 09:02:33 CSTGO:2000866 positive regulation of estradiol secretion (P)PMID:24139936ECO:0000314 direct assay evidence used in manual assertion

Figure 4.

challenge
acceptablePIG:IL1BDrl5141, Team Go Pro2013-11-04 08:58:57 CSTGO:2000866 positive regulation of estradiol secretion (P)PMID:24139936ECO:0000314 direct assay evidence used in manual assertion

Day 15 and 16 of Figure 4 show that increased IL-1beta resulted in an increase in estradiol 17 beta

challenge
acceptablePIG:IL6Drl5141, Team Go Pro2013-11-04 08:55:36 CSTGO:2000866 positive regulation of estradiol secretion (P)PMID:24139936ECO:0000314 direct assay evidence used in manual assertion

Day 15 and 16 of Pregnancy figure 4 show that increased IL-6 leads to increased estradiol 17beta secretion.

challenge
acceptableHUMAN:RFIP5Sabrinamoisan, Team Go Pro2013-10-22 20:27:46 CDTGO:0070164 negative regulation of adiponectin secretion (P)PMID:24040321ECO:0000314 direct assay evidence used in manual assertion

Figure 1A

challenge
acceptableHUMAN:RFIP3Sabrinamoisan, Team Go Pro2013-10-22 20:26:23 CDTGO:0070164 negative regulation of adiponectin secretion (P)PMID:24040321ECO:0000314 direct assay evidence used in manual assertion

Figure 1A

challenge
acceptableHUMAN:RFIP1Sabrinamoisan, Team Go Pro2013-10-22 20:25:26 CDTGO:0070164 negative regulation of adiponectin secretion (P)PMID:24040321ECO:0000314 direct assay evidence used in manual assertion

Figure 1A

challenge
acceptableMOUSE:ZBT20Drl5141, Team Go Pro2013-10-08 20:22:58 CDTGO:0045892 negative regulation of transcription, DNA-dependent (P)PMID:23776228ECO:0000314 direct assay evidence used in manual assertion

In fig. 6A HEK293 cells were cotransfected with ZBTB20 expressing plasmids while maintaining a constant amount of DNA transfected versus mock normal DNA shows that in this repression fold graph that when the cotransfected plasmid is at its largest the amount of transcription is decreased 10 fold of expression. This amount transcription is decreased is in correlation to the amount of ZBT20 plasmid present.

challenge
acceptableMOUSE:TRAIPDrl5141, Team Go Pro2013-10-08 19:28:27 CDTGO:0032688 negative regulation of interferon-beta production (P)PMID:22945920ECO:0000314 direct assay evidence used in manual assertion

Figure 1.E TRIP expression decreased overall amount of interferon being made when compared to control thus indicating that TRAF interacting protein negatively regulates interferon-beta production.

challenge
acceptableMOUSE:ZFP64Drl5141, Team Go Pro2013-10-08 19:18:49 CDTGO:0032755 positive regulation of interleukin-6 production (P)PMID:23857586ECO:0000314 direct assay evidence used in manual assertion

Elisa after overexpression of ZFP64 in RAW264.7 cells shows in figure 2A graph 2 that interleukin-6 (IL-6) production has been increased from the normal Raw264.7 cells indicating ZFP64 positively regulates interleukin-6 production.

challenge
acceptableMOUSE:ZFP64Drl5141, Team Go Pro2013-10-08 19:18:49 CDTGO:0032755 positive regulation of interleukin-6 production (P)PMID:23857586ECO:0000315 mutant phenotype evidence used in manual assertion

Elisa was run after using siRNA to decrease expression of ZFP64 resulting in Figure 2B graph 2 showing decreased expression of Interleukin-6 indicating that ZFP64 positively regulates Interleukin-6 production.

challenge
acceptableMOUSE:ZFP64Drl5141, Team Go Pro2013-10-08 19:18:49 CDTGO:0032728 positive regulation of interferon-beta production (P)PMID:23857586ECO:0000314 direct assay evidence used in manual assertion

Figure 2A graph 3 of ELISA shows when ZFP64 is overexpressed, interferon beta production increases thus indicating that ZFP64 positively regulates interferon-beta production inRAW264.7 cells.

challenge
acceptableMOUSE:ZFP64Drl5141, Team Go Pro2013-10-08 19:18:49 CDTGO:0032728 positive regulation of interferon-beta production (P)PMID:23857586ECO:0000315 mutant phenotype evidence used in manual assertion

In figure 2B graph 3 elisa when siRNA was insereted to decrease ZFP64 expression shows that interferon-beta production decreases indicating that ZFP64 positively regulates interferon-beta production.

challenge
acceptableMOUSE:ZFP64Drl5141, Team Go Pro2013-10-08 18:59:20 CDTGO:0032760 positive regulation of tumor necrosis factor production (P)PMID:23857586ECO:0000314 direct assay evidence used in manual assertion

The overexpression of ZFP64 and stimulated with LPS resulted in increased amounts of Tumor necrosis Factor production alpha as shown in Figure 2 A graph 1 elisa indicating that ZFP64 is a positive regulator of tumor necrosis factor production in RAW264.7 cells.

challenge
acceptableMOUSE:ZFP64Drl5141, Team Go Pro2013-10-08 18:59:20 CDTGO:0032760 positive regulation of tumor necrosis factor production (P)PMID:23857586ECO:0000315 mutant phenotype evidence used in manual assertion

In mouse macrophages siRNA was used to decrease ZFP64 production thus resulting in Figure 2B graph 1 showing with stimulation by LPS decreased amounts of Tumor Necrosis Factor Alpha is made thus showing that ZFP64 when present is a positive regulator of tumor necrosis factor alpha production.

challenge
updatedbyinstructorHUMAN:BUB1Sabrinamoisan, Team Go Pro2013-09-24 20:50:47 CDTGO:0000942 condensed nuclear chromosome outer kinetochore (C)PMID:24055156ECO:0000314 direct assay evidence used in manual assertion

Figure S1A. The authors state that "Sgo1 and Bub1 localized to inner centromeres and outer kinetochores, respectively.

challenge
acceptableHUMAN:SGOL1Sabrinamoisan, Team Go Pro2013-09-24 20:44:47 CDTGO:0000775 chromosome, centromeric region (C)PMID:24055156ECO:0000314 direct assay evidence used in manual assertion

Figure 1A

challenge
acceptableMOUSE:ZBT20Drl5141, Team Go Pro2013-09-24 20:37:01 CDTGO:0010629 negative regulation of gene expression (P)PMID:23776228ECO:0000315 mutant phenotype evidence used in manual assertion

In fig. 4A and S6 when the control is compared to knockout ZBT20 in immunoblot analysis, the IkB alpha gene shows increased expression of IkBalpha so when Zbt20 is present IkB alph gene transcription is repressed.

challenge
acceptableMOUSE:ZBT20Drl5141, Team Go Pro2013-09-24 20:09:28 CDTGO:0032755 positive regulation of interleukin-6 production (P)PMID:23776228ECO:0000314 direct assay evidence used in manual assertion

Figure 3E graph 2 demonstrates that when Zbt20 is overexpressed there is an increased production of interleukin-6 (IL-6) in response when compared to the control.

challenge
acceptableMOUSE:ZBT20Drl5141, Team Go Pro2013-09-24 20:07:28 CDTGO:0032760 positive regulation of tumor necrosis factor production (P)PMID:23776228ECO:0000314 direct assay evidence used in manual assertion

Figure 3E graph 1 demonstrates when zbt20 is overexpressed there is an increased amount of TNF (tumor necrosis factor) produced when compared to the control.

challenge
acceptableMOUSE:ZBT20Drl5141, Team Go Pro2013-09-24 20:04:15 CDTGO:0032728 positive regulation of interferon-beta production (P)PMID:23776228ECO:0000314 direct assay evidence used in manual assertion

Overexpression of Zbt20 results in figure 3E graph 3, with excess zbt20 expression there is an increased production of interferon beta.

challenge
acceptableMOUSE:ZBT20Drl5141, Team Go Pro2013-09-24 19:57:16 CDTGO:0032755 positive regulation of interleukin-6 production (P)PMID:23776228ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1A the third graph with the knockout of the protein Zbt20 shows a lowered amount of interleukin 6 (IL-6) being produced. This is also shown in figure 3A second graph and figure 3c graph 2 with siRNA with zbt20 resulting in lowered quantities of IL-6.

challenge
updatedbyinstructorMOUSE:HYAS3Sabrinamoisan, Team Go Pro2013-09-24 19:56:46 CDTGO:0005737 cytoplasm (C)PMID:24057227ECO:0000314 direct assay evidence used in manual assertion

Figures 1,2,3,4

challenge
updatedbyinstructorMOUSE:HYAS2Sabrinamoisan, Team Go Pro2013-09-24 19:55:06 CDTGO:0005737 cytoplasm (C)PMID:24057227ECO:0000314 direct assay evidence used in manual assertion

Figures 1,2,3,4

challenge
updatedbyinstructorMOUSE:HYAS1Sabrinamoisan, Team Go Pro2013-09-24 19:53:40 CDTGO:0005737 cytoplasm (C)PMID:24057227ECO:0000314 direct assay evidence used in manual assertion

Figures 1, 2, 3, 4

challenge
acceptableMOUSE:ZBT20Drl5141, Team Go Pro2013-09-24 19:51:15 CDTGO:0032760 positive regulation of tumor necrosis factor production (P)PMID:23776228ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1A the first graph with the knockout of the protein shows Zbt20 shows that TNF is present in significantly lowered quantities. This is also shown in figure 3A first graph and figure 3c graph 1 with RNA interference of zbt20 production resulting in lowered amount of TNF.

challenge
acceptableMOUSE:ZBT20Drl5141, Team Go Pro2013-09-24 19:47:43 CDTGO:0032728 positive regulation of interferon-beta production (P)PMID:23776228ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1A the third graph with the knockout of the protein zbt20 shows IFN beta in significantly lowered quanities. This is also shown in figure 3A third graph and figure 3c graph 3 with siRNA interference of ZBT20 resulting in similar effects.

challenge
acceptableHUMAN:IFT81Sabrinamoisan, Team Go Pro2013-09-24 19:22:05 CDTGO:0042384 cilium assembly (P)PMID:23990561ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2

challenge
acceptableHUMAN:IFT81Sabrinamoisan, Team Go Pro2013-09-24 19:22:05 CDTGO:0036064 cilium basal body (C)PMID:23990561ECO:0000314 direct assay evidence used in manual assertion

Figure S6A

challenge
acceptableBOMMO:Q5CCJ5Sabrinamoisan, Team Go Pro2013-09-22 23:42:55 CDTGO:0005634 nucleus (C)PMID:23781494ECO:0000314 direct assay evidence used in manual assertion

Figure 5

challenge
acceptableBOMMO:Q5CCJ5Sabrinamoisan, Team Go Pro2013-09-22 23:42:55 CDTGO:0005737 cytoplasm (C)PMID:23781494ECO:0000314 direct assay evidence used in manual assertion

Figure 5

challenge
acceptableHUMAN:ANM6Sabrinamoisan, Team Go Pro2013-09-15 14:38:09 CDTGO:0008469 histone-arginine N-methyltransferase activity (F)PMID:23980157ECO:0000314 direct assay evidence used in manual assertion

Figure 2

challenge
acceptableHUMAN:CARM1Sabrinamoisan, Team Go Pro2013-09-15 14:33:03 CDTGO:0008469 histone-arginine N-methyltransferase activity (F)PMID:23980157ECO:0000314 direct assay evidence used in manual assertion

Figure 2

challenge
unacceptableCHLRE:Q68RJ5Sabrinamoisan, Team Go Pro2013-09-15 14:10:50 CDTGO:0036064 cilium basal body (C)PMID:23990561ECO:0000314 direct assay evidence used in manual assertion

Figure S6A

challenge
acceptableMOUSE:KSYKDrl5141, Team Go Pro2013-09-15 13:39:50 CDTGO:0002092 positive regulation of receptor internalization (P)PMID:23962979ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 1.E and 1.D Internalization of TLR4 by LPS mediated endocytosis via CD14 amounts was increased in presence of Syk compared to mutant.

challenge
requireschangesHUMAN:KSYKDrl5141, Team Go Pro2013-09-15 12:35:47 CDTGO:0006468 protein phosphorylation (P)PMID:19151749ECO:0000314 direct assay evidence used in manual assertion

Fig 1.C Syk phosphorylated TRIP.

challenge
requireschangesHUMAN:KSYKDrl5141, Team Go Pro2013-09-15 12:32:29 CDTGO:0006468 protein phosphorylation (P)PMID:19151749ECO:0000315 mutant phenotype evidence used in manual assertion

1.B Syk phosphorylates TRIP.

challenge
requireschangesHUMAN:KSYKDrl5141, Team Go Pro2013-09-15 12:25:00 CDTGO:1901224 positive regulation of NIK/NF-kappaB cascade (P)PMID:19151749ECO:0000314 direct assay evidence used in manual assertion

Fig 1.B Increased TNF signaling by Syk here demonstrates increased NF-kB activation.

challenge
unacceptableMOUSE:TRIPCDrl5141, Team Go Pro2013-09-13 09:18:14 CDTGO:0032688 negative regulation of interferon-beta production (P)PMID:22945920ECO:0000314 direct assay evidence used in manual assertion

Figure 1.E and 1.F, TRIP expression decreased overall amount of interferon being made when compared to control.

challenge
unacceptableCHLRE:Q68RJ5Sabrinamoisan, Team Go Pro2013-09-10 20:43:44 CDTGO:0042384 cilium assembly (P)PMID:23990561ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2

challenge
acceptableHUMAN:ZFY27Sabrinamoisan, Team Go Pro2013-09-10 19:41:49 CDTGO:0071782 endoplasmic reticulum tubular network (C)PMID:23969831ECO:0000314 direct assay evidence used in manual assertion

Figure 1

challenge

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Pages in category "Team Go Pro"

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