User:MAntonescu

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	CAUVC:Q9A912	2019-01-29 15:54:34 CST	GO:0000160 phosphorelay signal transduction system (P)	PMID:16176121	ECO:0001202 in vitro protein kinase assay evidence used in manual assertion	Figure 5 B shows phosphorelay signal transduction system in C Crescentus from the kinase CC1181 to CC1182. The histidine kinase autophosphorylates. The phosphoryl group is then passed to the receiver domain of a cognate response regulator. Phosphorylation of the receiver domain typically activates the output domain.	challenge
updatedbyinstructor	LAMBD:SPAN1	2019-02-05 15:19:08 CST	GO:0061025 membrane fusion (P)	PMID:25870259	ECO:0007122 in vitro assay evidence used in manual assertion	Figure 3C shows the spheroplast fusion assay in lambda, in which inner membrane protein Rz and outer membrane protein Rz1 fuse together during the last step of the lytic stage. Green GFP displays Rz and red mCherry displays Rz1.	challenge
acceptable	LAMBD:SPAN2	2019-02-05 15:22:19 CST	GO:0061025 membrane fusion (P)	PMID:25870259	ECO:0007122 in vitro assay evidence used in manual assertion	Figure 3C shows the spheroplast fusion assay in lambda, in which inner membrane protein Rz and outer membrane protein Rz1 fuse together during the last step of the lytic stage. Green GFP displays Rz and red mCherry displays Rz1.	challenge
acceptable	LAMBD:FIBER	2019-02-12 15:48:26 CST	GO:0046813 receptor-mediated virion attachment to host cell (P)	PMID:30463036	ECO:0007042 epifluorescence microscopy evidence used in manual assertion	Figure 3B shows epifluorescence microscopy evidence used in manual assertion in bacteriophage lambda. Stf, the tail fiber protein, enhances adsorption of ur lambda, causing less darker infection frequencies	challenge
unacceptable	LAMBD:FIBER	2019-02-12 16:14:40 CST	GO:0098689 latency-replication decision (P)	PMID:30463036	ECO:0006037 genetically encoded fluorescent sensor evidence used in manual assertion	Figure 4B and C show the effect of stf (tail fiber protein) on Ur-λ post-infection in bacteriophage lambda. Lysogenization probability of Ur-λ is higher than λWT. This probability increases with increased MOIs.	challenge
unacceptable	BPP2:SPIKE	2019-03-12 20:34:16 CDT	GO:0019062 virion attachment to host cell (P)	PMID:19780551	ECO:0000247 sequence alignment evidence used in manual assertion	Tail spikes in Escherichia phage P2 (Bacteriophage P2) is composed of gpV. In Fig 9 B, P2 gpV gets compared to Mu gp45. The results indicate that P2 gpV has typical features of phage tail spikes, such as a protease-sensitive N-terminal domain, a host-binding C-terminal domain, and a trimeric structure. This shows that gpV is a receptor-binding protein involved in the second step of phage adsorption.	challenge
unacceptable	BPP2:SPIKE	2019-03-12 20:40:13 CDT	GO:0019062 virion attachment to host cell (P)	PMID:19780551	ECO:0000333 sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence	Tail spikes in Escherichia phage P2 (Bacteriophage P2) are composed of gpV. In Fig 7, gpV-C was found in the precipitant with the membrane when purified gpV-C and E. coli membranes were mixed and centrifuged. This suggests that gpV-C bound to and precipitated with the E. coli membranes. No used in manual assertion child term.	challenge
unacceptable	LEPIR:Q93MT8	2019-03-19 16:07:04 CDT	GO:0110001 toxin-antitoxin complex (C)	PMID:25461800	ECO:0001258 spectrophotometry evidence used in manual assertion	Figure 1B show growth curves, in which OD600 reflect the number of E. coli in cultures incubated. ChpK, also called mRNA interferase, is a toxin in Leptospira interrogans that allows increase in OD600 as long as its expressed alongside the antoxin ChpI that neutralizes the toxin. If ChpK is expressed without the ChpI, the toxicity of ChpK will cause the OD600 value to decrease.	challenge
unacceptable	LEPIR:Q93MT9	2019-03-19 16:08:53 CDT	GO:0110001 toxin-antitoxin complex (C)	PMID:25461800	ECO:0001258 spectrophotometry evidence used in manual assertion	Figure 1B show growth curves, in which OD600 reflect the number of E. coli in cultures incubated. ChpI is a antitoxin in Leptospira interrogans that allows increase in OD600 if by itself or if its expressed alongside the toxin ChpK, in which case it neutralizes the toxin.	challenge

acceptable:3
unacceptable:5
requires_changes:0
flagged:0

Annotations challenged by MAntonescu

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
unacceptable	Mason.Tomas, Catalyzers	BORBU:O50835	GO:0045959 - negative regulation of complement activation, classical pathway (P)	PMID:26808924	ECO:0000279 qualitative western immunoblotting evidence used in manual assertion	challenge	C: 2
unacceptable	ADPowell, Catalyzers	BORBG:C5J482	GO:0004867 - serine-type endopeptidase inhibitor activity (F)	PMID:26808924	ECO:0001269 surface plasmon resonance evidence used in manual assertion	challenge	C: 1

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