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LAMBD:SPAN2
Contents
| Species (Taxon ID) | Escherichia phage lambda (Bacteriophage lambda). (10710) | |
| Gene Name(s) | Rz1 | |
| Protein Name(s) | Spanin, outer lipoprotein subunit
o-spanin Outer membrane lipoprotein Rz1 | |
| External Links | ||
| UniProt | Q37935 | |
| EMBL | J02459 U37314 | |
| PIR | JN0750 | |
| RefSeq | YP_001551744.1 | |
| IntAct | Q37935 | |
| TCDB | 1.M.1.1.1 | |
| GeneID | 5739319 | |
| KEGG | vg:5739319 | |
| Proteomes | UP000001711 | |
| GO | GO:0039662 GO:0020002 GO:0016020 GO:0019835 GO:0019076 | |
| InterPro | IPR010346 | |
| Pfam | PF06085 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
| GO:0039662 |
host cell outer membrane |
ECO:0000314 |
C |
Figure 6.B. lane 3 deomonstrates lambda Rzl localizes in the host cell outer membrane. |
complete | |||||
| GO:0042597 |
periplasmic space |
ECO:0000314 |
C |
Figure 2. "Purification of the Rz and Rz1 periplasmic domains" |
complete | |||||
| GO:0019076 |
cytolysis |
ECO:0000314 |
P |
Only the C-terminal R153 residue is dispensable; there are lysis-defective nonsense mutations at positions 151 and 152 shown (Table 3) in the CTD region. A nonsense mutation (Rz C152X) shows the same effect as lysogen carrying an empty pRE plasmid vector (Vector), inhibiting cytolysis (Figure 3). The wild type pRz (WT), pRz with an artificial transmembrane domain (ART-TMD), and pRz 100–115 (Ser-Gly) show effective cytolysis in Figure 3. |
complete | |||||
| GO:0090680 |
disruption by virus of host outer membrane |
ECO:0000315 |
P |
Fig. 2a,b,e and f demonstrates that Rz1, a spanin outer lipoprotein subunit of the Escherichia phage lambda is essential part of the RzRz1 spanin complex, which rapidly lyses the E. coli bacterium. When mutant was observed, the outer membrane was not ruptured, preventing lysis of the bacterium. |
complete | |||||
|
part_of |
GO:0039662 |
host cell outer membrane |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0019076 |
viral release from host cell |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0019835 |
cytolysis |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0020002 |
host cell plasma membrane |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0033644 |
host cell membrane |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0016020 |
membrane |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0019835 |
cytolysis |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
| GO:0061025 |
membrane fusion |
ECO:0007122 |
P |
Figure 3C shows the spheroplast fusion assay in lambda, in which inner membrane protein Rz and outer membrane protein Rz1 fuse together during the last step of the lytic stage. Green GFP displays Rz and red mCherry displays Rz1. |
complete | |||||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ 1.0 1.1 Kedzierska, S et al. (1996) The Rz1 gene product of bacteriophage lambda is a lipoprotein localized in the outer membrane of Escherichia coli. Gene 168 1-8 PubMed GONUTS page
- ↑ Berry, J et al. (2010) The lambda spanin components Rz and Rz1 undergo tertiary and quaternary rearrangements upon complex formation. Protein Sci. 19 1967-77 PubMed GONUTS page
- ↑ Cahill, J et al. (2017) Genetic Analysis of the Lambda Spanins Rz and Rz1: Identification of Functional Domains. G3 (Bethesda) 7 741-753 PubMed GONUTS page
- ↑ Berry, J et al. (2012) The spanin complex is essential for lambda lysis. J. Bacteriol. 194 5667-74 PubMed GONUTS page
- ↑ Rajaure, M et al. (2015) Membrane fusion during phage lysis. Proc. Natl. Acad. Sci. U.S.A. 112 5497-502 PubMed GONUTS page
