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GO:0019835cytolysisPMID:28040784IMP: Inferred from Mutant Phenotype P
This annotation made on page: LAMBD:SPAN2
By: ORichter (group Team Purple B 2018) on 2018-03-04 19:10:43 CST.




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
Public
Assessment
Scaruso2018-03-22 19:48:52 CDTNo notes given.Acceptable
Protein
Publication
Qualifier
Go term
Evidence
With/From
Notes
Unique/Original
Public
Assessment
DanielRenfro2018-03-11 22:48:56 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0019076 cytolysis PMID:28040784 IDA: Inferred from Direct Assay P Only the C-terminal R153 residue is dispensable; there are lysis-defective nonsense mutations at positions 151 and 152 shown (Table 3) in the CTD region. A nonsense mutation (Rz C152X) shows the same effect as lysogen carrying an empty pRE plasmid vector (Vector), inhibiting cytolysis (Figure 3). The wild type pRz (WT), pRz with an artificial transmembrane domain (ART-TMD), and pRz 100–115 (Ser-Gly) show effective cytolysis in Figure 3. complete
CACAO 13098
on LAMBD:SPAN2
Flagged
Public
Assessment
Scaruso2018-03-08 11:45:24 CST

Your note is extensive. It includes explanatory background, which is fine, but not always necessary, but doesn't have what you really need: How does a particular experiment demonstrate that we know the gene does something. So, you are annotating (claiming) that the paper says the gene produces a protein that causes cytolysis, and that we can tell from a mutation experiment. Which figure demonstrates that and how? Figure 1 is a background figure and figure 2 is showing where the mutations are. You want to use one that allows you to "IMP: Inferred from Mutant Phenotype" see a phenotype resulting from the mutation. You don't need to describe the whole paper, just the figure that lets you do that. You might also be able to come up with a better GO term. Check out GO:0019076. You might like it or one of its synonyms better.

Requires Changes
Protein
Publication
Qualifier
Evidence
With/From
Notes
Unique/Original