Cacao

• Correction to Defense: Figure 7 does *not show protein localization to this domain.

DEFENSE: figure 7 only shows the dimensions of the periplasmic space, and does show protein localization in this domain. "Determination of periplasmic dimensions using cryo-electron microscopy. E. coli K-12 cells in logarithmic phase were plunged into liquid ethane and imaged by cryo-electron microscopy. Shown is a representative whole, frozen-hydrated cell with an inset box indicating the region of interest. The average distance between the inner and outer membranes at the mid-length of 31 such cells was 25.5 ± 3.1 nm. The periplasmic distance increases at the poles as shown in this example"

While a GO-term describing protein localization to the outer membrane is fitting to this protein, this paper shows it has a domain in the periplasm...which is what the annotation is describing. A second annotation from a separate paper would be needed to properly demonstrate and annotate protein localization to the outer membrane.

Figure 2 shows the purification of the proteins. The actual periplasmic localization of the Rz and Rz1 domains is seen in figure 7. A better GO term would be: 0089705: Protien localization to outer membrane