GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:30463036
| Citation |
Guan, J, Ibarra, D and Zeng, L (2019) The role of side tail fibers during the infection cycle of phage lambda. Virology 527:57-63 |
|---|---|
| Abstract |
Bacteriophage λ has served as an important model for molecular biology and different cellular processes over the past few decades. In 1992, the phage strain used in most laboratories around the world, thought of as λ wild type, was discovered to carry a mutation in the stf gene which encodes four side tail fibers. Up to now, the role of the side tail fibers during the infection cycle, especially at the single-cell level, remains largely unknown. Here we utilized fluorescent reporter systems to characterize the effect of the side tail fibers on phage infection. We found that the side tail fibers interfere with phage DNA ejection process, most likely through the binding with their receptors, OmpC, leading to a more frequent failed infection. However, the side tail fibers do not seem to affect the lysis-lysogeny decision-making or lysis time. |
| Links |
PubMed PMC6312755 Online version:10.1016/j.virol.2018.11.005 |
| Keywords |
|
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0001618: virus receptor activity |
ECO:0007042: |
F |
Fig. 3B shows that ompC in E.coli is involved in the adsorption of the side tail fiber (stf) of bacteriophage lambda. |
complete | ||||
| GO:0046813: receptor-mediated virion attachment to host cell |
ECO:0007042: epifluorescence microscopy evidence used in manual assertion |
P |
Figure 3B shows that the presence of the ompC receptor in E. Coli causes the side tail fibers to strongly adsorb, which leads to less dark infection. |
complete | ||||
| GO:0046813: receptor-mediated virion attachment to host cell |
ECO:0007042: |
P |
Figure 3B shows epifluorescence microscopy evidence used in manual assertion in bacteriophage lambda. Stf, the tail fiber protein, enhances adsorption of ur lambda, causing less darker infection frequencies |
complete | ||||
| GO:0098689: latency-replication decision |
ECO:0006037: |
P |
Figure 4B and C show the effect of stf (tail fiber protein) on Ur-λ post-infection in bacteriophage lambda. Lysogenization probability of Ur-λ is higher than λWT. This probability increases with increased MOIs. |
complete | ||||
Notes
See also
References
See Help:References for how to manage references in GONUTS.
