User:Lesliea2

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	ECOLI:AQPZ	2012-04-10 12:04:12 CDT	GO:0006833 water transport (P)	PMID:16385074	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 1: The osmotic permeability is much greater in the wild-type(Fig.1A) strain compared to the (ΔaqpZ::Cam+) strain (Fig.1B).	challenge
requireschanges	MYCSM:DDL	2012-04-15 13:27:46 CDT	GO:0008716 D-alanine-D-alanine ligase activity (F)	PMID:149019	ECO:0000314 direct assay evidence used in manual assertion	Figure 3A: Analysis of the production of the D-Alanine:D-Alanine dipeptide by different strains. Strains GPM259, GPM260, and GPM265 all show overproduction of the ligase due to amplification of the ddl gene compared to strains mc2155 and GPM14 which do not carry this amplification.	challenge
requireschanges	MYCSM:ALR	2012-04-15 15:26:26 CDT	GO:0008784 alanine racemase activity (F)	PMID:9260945	ECO:0000314 direct assay evidence used in manual assertion	Figure 5: Depicts alanine racemase activity among different strains. Strain GPM14 shows marked overproduction of d-alanine racemase as a result of a single base change from T -> G in the alrA gene promoter as described in the "Results" section.	challenge
acceptable	BACLI:AMY	2012-04-15 17:18:16 CDT	GO:0004556 alpha-amylase activity (F)	PMID:2540150	ECO:0000314 direct assay evidence used in manual assertion	Figure 1: The alpha-amylase is measured from B. licheniformis grown on media with starch and with or without glucose. Alpha-amylase activity greatly increased when starch was the main carbon source.	challenge

acceptable:2
unacceptable:0
requires_changes:2
flagged:0

Annotations challenged by Lesliea2

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
requireschanges	Rolfecat, Team Grizzly Ninjas	PENCH:AAAA	GO:0050640 - isopenicillin-N N-acyltransferase activity (F)	PMID:8344300	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 1

0 annotations fixed by Lesliea2

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
requireschanges	ECOLI:CSGD	2012-03-28 22:56:55 CDT	GO:0042710 biofilm formation (P)	PMID:17873038	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 1: curli fiber production due to increased penicillin production after plasmid with CsgD gene introduced.	challenge
requireschanges	PSESD:ATZB	2012-04-01 14:31:58 CDT	GO:0018763 hydroxydechloroatrazine ethylaminohydrolase activity (F)	PMID:9055410	ECO:0000314 direct assay evidence used in manual assertion	Figure 2: N-isopropylammelide was further degraded by crude extracts from Pseudomonas aeruginosa, but not by those of E. coli with pMD1 and pATZB-2 plasmids. N-isopropylammelide is an intermediate of the breakdown of atrazine.	challenge
acceptable	PSEAE:TOXA	2012-04-01 15:59:24 CDT	GO:0047286 NAD+-diphthamide ADP-ribosyltransferase activity (F)	PMID:2170123	ECO:0000314 direct assay evidence used in manual assertion	Figure 3: ADP-ribosylation activity of exotoxin A with NAD+ after 30 minutes.	challenge

acceptable:1
unacceptable:0
requires_changes:2
flagged:0

Annotations challenged by Lesliea2

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
requireschanges	Rolfecat, Team Grizzly Ninjas	PENCH:AAAA	GO:0050640 - isopenicillin-N N-acyltransferase activity (F)	PMID:8344300	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 1

0 annotations fixed by Lesliea2