SUPER BOWL (Round 5)
My Annotations
| Status | Page | Date/Time | GO Term (Aspect) | Reference | Evidence | Notes | Links |
|---|
| requireschanges | ZOBGA:Q9RGX9 | 2012-04-14 15:38:39 CDT | GO:0033916 beta-agarase activity (F) | PMID:15456406 | ECO:0000314 direct assay evidence used in manual assertion | Assists in cleaving the β-(1→4) linkages of agarose.
Figure 3 shows the hydrolysis of agarose by AgaA. After 24 hours,neoagarotetraose and neoagarohexaose were produced.
| challenge |
| requireschanges | IDEDE:CLRA | 2012-04-14 15:53:52 CDT | GO:0047143 chlorate reductase activity (F) | PMID:12957948 | ECO:0000314 direct assay evidence used in manual assertion | ClrA is one of three subunits which contribute to the chlorate reductase activity.
Figure 1 uses SDS-PAGE to purify the chlorate reductase. The results were 3 major bands which shows it is a complex of 3 different subunits in a 1:1:1 ratio.
| challenge |
| requireschanges | 9PSED:Q7WSJ4 | 2012-04-14 16:17:20 CDT | GO:0034013 aliphatic aldoxime dehydratase activity (F) | PMID:12773527 | ECO:0000314 direct assay evidence used in manual assertion | OxdA encodes for aldoxime dehydratase activity.
Figure 3As shows the activity between the purified enzyme, the enzyme with Na2S2O4-reduced, and the enzyme with Na2S2O4-reduced plus CO. As a result, oxdA was shown to have much higher activity in the presence of Na2S2O4. This shows the Na2S2O4 reagent assists in the already present reducing activity.
| challenge |
| requireschanges | PSEOL:RURE | 2012-04-14 16:56:12 CDT | GO:0015044 rubredoxin-NAD+ reductase activity (F) | PMID:10727934 | ECO:0000314 direct assay evidence used in manual assertion | alkT encodes the 3rd component of the alkane hydroxylase complex, the rubredoxin reductase. Transfers electrons from NADH to rubredoxin.
Figure 7 shows the highest specific activity for alkT in P.oleovorans, lower in E.coli HB101 and lowest in E.coli W3110. This concluded that the rubredoxin reductase is present
in excess in the E. coli alk+ recombinants
| challenge |
| requireschanges | CUPPJ:TFDA | 2012-04-14 17:54:42 CDT | GO:0018602 2,4-dichlorophenoxyacetate alpha-ketoglutarate dioxygenase activity (F) | PMID:3036764 | ECO:0000314 direct assay evidence used in manual assertion | TfdA codes for the 2,4-D monooxygenase.
Figure 4 shows omega mutagenesis of the tfdA gene. An omega fragment containing stop signals was inserted into the coding region of a tfdA plasmid. The results were a truncated tfdA product for the plasmids which the omega fragment was inserted, in turn disrupting the enzymatic activity for the 2,4-D monooxygenase.
| challenge |
| requireschanges | PSEMV:MVAA | 2012-04-15 12:58:38 CDT | GO:0042282 hydroxymethylglutaryl-CoA reductase activity (F) | PMID:2656635 | ECO:0000314 direct assay evidence used in manual assertion | mvaA codes for the 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase.
Figure 5 shows E.coli cells transformed with the plasmid containing mvaA and induced with IPTG. Assayed for HMG-CoA reductase activity. Cells induced with IPTG resulted in HMG-CoA reductase activity shown in lane 1.
| challenge |
| requireschanges | ECOLI:FABH | 2012-04-15 13:04:52 CDT | GO:0004315 3-oxoacyl-(acyl-carrier-protein) synthase activity (F) | PMID:1551888 | ECO:0000314 direct assay evidence used in manual assertion | FabH catalyzes the condensation of acetyl-CoA and initiation of fatty acid synthesis.
Table 2 shows the level of FabH correlates with the membrane fatty acid composition. Chromatography was used to analyze the fatty acid methyl esters.
| challenge |
| requireschanges | PSESD:ATZC | 2012-04-15 13:21:23 CDT | GO:0018764 N-isopropylammelide isopropylaminohydrolase activity (F) | PMID:9422605 | ECO:0000314 direct assay evidence used in manual assertion | AtzC codes for the enzyme which transforms N-isopropylammelide to cyanuric acid and isopropylamine by hydrolytic deamination.
Table 1 shows the control strain of Pseduomonas had this activity. The E.coli strains which had a plasmid insterted containing the AtzC gene also transformed N-isopropylammelide to cyanuric acid and isopropylamine.
| challenge |
acceptable:0
unacceptable:0
requires_changes:8
flagged:0
Annotations challenged by Rolfecat
| Status | Author,Group | Page | GO Term (Aspect) | Reference | Evidence | Links | Page history |
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0 annotations fixed by Rolfecat
MSU Internal
My Annotations
| Status | Page | Date/Time | GO Term (Aspect) | Reference | Evidence | Notes | Links |
|---|
| requireschanges | PENCH:AAAA | 2012-04-01 16:05:09 CDT | GO:0050640 isopenicillin-N N-acyltransferase activity (F) | PMID:8344300 | ECO:0000314 direct assay evidence used in manual assertion | Responsible for isopenicillin-N amidohydrolase, 6-aminopenicillanic acid acyltransferase and penicillin amidase activities in P. chrysogenum.
Mutants npe6, npe8 and npe10 with no acyltransferase activity were transformed with a plamsid, pULB101, containing penDE gene. Mutants were then prepared and assayed. Npe6, npe8 and npe10 showed AAT, IAT, IPN amidohydrolase, penicillin transacylase and penicllin amidase activities. This indicated the penDE gene recovered all acyltransferase activity. Table 6 shows the results of an assay for the comparison between P. chrysogenum and C. acremonium. C. acremonium does not contain the penDE gene and recorded zero activity for Acyl-CoA:CAPA acyltransferase activity, extracellular accumulation of 6-APA, and production of penicillin. Whereas, P. chrysogenum does contain the penDE gene and showed positive results for these activities.
| challenge |
| requireschanges | BACGL:UGL | 2012-03-31 17:37:50 CDT | GO:0052788 d-4,5 unsaturated beta-glucuronyl hydrolase activity (F) | PMID:10441389 | ECO:0000314 direct assay evidence used in manual assertion | Release of unsaturated glucuronic acids from oligosaccharides produced from polysaccharide lyase reactions.
Fig. 2 shows the transformation from a tetrasaccharide to a trisaccharide without unsaturated glucuronic acid using enzyme fraction. This shows an enzyme, unsaturated
glucuronyl hydrolase, is present in Bacillus sp. GL1. Also, the absorbance level at 235nm was shown to decrease due to the double bond diminishing over a 15min period.
| challenge |
acceptable:0
unacceptable:0
requires_changes:2
flagged:0
Annotations challenged by Rolfecat
| Status | Author,Group | Page | GO Term (Aspect) | Reference | Evidence | Links | Page history |
|---|
0 annotations fixed by Rolfecat
