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PMID:12957948
| Citation |
Thorell, HD, Stenklo, K, Karlsson, J and Nilsson, T (2003) A gene cluster for chlorate metabolism in Ideonella dechloratans. Appl. Environ. Microbiol. 69:5585-92 |
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| Abstract |
Chlorate reductase has been isolated from the chlorate-respiring bacterium Ideonella dechloratans, and the genes encoding the enzyme have been sequenced. The enzyme is composed of three different subunits and contains molybdopterin, iron, probably in iron-sulfur clusters, and heme b. The genes (clr) encoding chlorate reductase are arranged as clrABDC, where clrA, clrB, and clrC encode the subunits and clrD encodes a specific chaperone. Judging from the subunit composition, cofactor content, and sequence comparisons, chlorate reductase belongs to class II of the dimethyl sulfoxide reductase family. The clr genes are preceded by a novel insertion sequence (transposase gene surrounded by inverted repeats), denoted ISIde1. Further upstream, we find the previously characterized gene for chlorite dismutase (cld), oriented in the opposite direction. Chlorate metabolism in I. dechloratans starts with the reduction of chlorate, which is followed by the decomposition of the resulting chlorite to chloride and molecular oxygen. The present work reveals that the genes encoding the enzymes catalyzing both these reactions are in close proximity. |
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| Keywords |
Amino Acid Sequence; Betaproteobacteria/classification; Betaproteobacteria/enzymology; Betaproteobacteria/genetics; Chlorates/metabolism; Isoenzymes/chemistry; Isoenzymes/genetics; Isoenzymes/metabolism; Molecular Sequence Data; Multigene Family; Mutagenesis, Insertional; Oxidation-Reduction; Oxidoreductases/chemistry; Oxidoreductases/genetics; Oxidoreductases/metabolism; Protein Subunits/chemistry; Protein Subunits/genetics; Protein Subunits/metabolism; Restriction Mapping; Sequence Alignment; Sequence Homology, Amino Acid |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
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Contributes to |
GO:0047143: chlorate reductase activity |
ECO:0000314: |
F |
ClrA is one of three subunits which contribute to the chlorate reductase activity. Figure 1 uses SDS-PAGE to purify the chlorate reductase. The results were 3 major bands which shows it is a complex of 3 different subunits in a 1:1:1 ratio. |
complete | |||
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