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PMID:10441389
| Citation |
Hashimoto, W, Kobayashi, E, Nankai, H, Sato, N, Miya, T, Kawai, S and Murata, K (1999) Unsaturated glucuronyl hydrolase of Bacillus sp. GL1: novel enzyme prerequisite for metabolism of unsaturated oligosaccharides produced by polysaccharide lyases. Arch. Biochem. Biophys. 368:367-74 |
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| Abstract |
The bacterium Bacillus sp. GL1 assimilates two kinds of heteropolysaccharides, gellan and xanthan, by using extracellular gellan and xanthan lyases, respectively, and produces unsaturated saccharides as the first degradation products. A novel unsaturated glucuronyl hydrolase (glycuronidase), which was induced in the bacterial cells grown on either gellan or xanthan, was found to act on the tetrasaccharide of unsaturated glucuronyl-glucosyl-rhamnosyl-glucose produced from gellan by gellan lyase, and the enzyme and its gene were isolated from gellan-grown cells. The nucleotide sequence showed that the gene contained an ORF consisting of 1131 base pairs coding a polypeptide with a molecular weight of 42,859. The purified enzyme was a monomer with a molecular mass of 42 kDa and was most active at pH 6.0 and 45 degrees C. Because the enzyme can act not only on the gellan-degrading product by gellan lyase, but also on unsaturated chondroitin and hyaluronate disaccharides produced by chondroitin and hyaluronate lyases, respectively, it is considered that the unsaturated glucuronyl hydrolase plays specific and ubiquitous roles in the degradation of oligosaccharides with unsaturated uronic acid at the nonreducing terminal produced by polysaccharide lyases. |
| Links |
PubMed Online version:10.1006/abbi.1999.1305 |
| Keywords |
Amino Acid Sequence; Bacillus/metabolism; Bacterial Proteins/metabolism; Base Sequence; Glycoside Hydrolases/metabolism; Hydrolases/metabolism; Molecular Sequence Data; Polysaccharide-Lyases/metabolism; Polysaccharides, Bacterial/metabolism; Substrate Specificity |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
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Contributes to |
GO:0052788: d-4,5 unsaturated beta-glucuronyl hydrolase activity |
ECO:0000314: |
F |
Release of unsaturated glucuronic acids from oligosaccharides produced from polysaccharide lyase reactions. Fig. 2 shows the transformation from a tetrasaccharide to a trisaccharide without unsaturated glucuronic acid using enzyme fraction. This shows an enzyme, unsaturated glucuronyl hydrolase, is present in Bacillus sp. GL1. Also, the absorbance level at 235nm was shown to decrease due to the double bond diminishing over a 15min period. |
complete | |||
See also
References
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