GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PSEOL:RURE

From GONUTS
Jump to: navigation, search
Species (Taxon ID) Pseudomonas oleovorans. (301)
Gene Name(s) alkT
Protein Name(s) Rubredoxin-NAD(+) reductase

RdxR

External Links
UniProt P17052
EMBL AJ245436
PIR S09114
ProteinModelPortal P17052
BioCyc MetaCyc:MONOMER-1021
UniPathway UPA00191
GO GO:0005737
GO:0050660
GO:0015044
GO:0015046
GO:0043448
GO:0045454
Gene3D 3.30.390.30
InterPro IPR016156
IPR013027
IPR004099
IPR023753
IPR001327
IPR028202
Pfam PF00070
PF07992
PF14759
PRINTS PR00368
SUPFAM SSF55424

Annotations

Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status

Contributes to

GO:0015044

rubredoxin-NAD+ reductase activity

PMID:10727934[1]

ECO:0000314

F

alkT encodes the 3rd component of the alkane hydroxylase complex, the rubredoxin reductase. Transfers electrons from NADH to rubredoxin.

Figure 7 shows the highest specific activity for alkT in P.oleovorans, lower in E.coli HB101 and lowest in E.coli W3110. This concluded that the rubredoxin reductase is present in excess in the E. coli alk+ recombinants

complete
CACAO 4718

enables

GO:0050660

flavin adenine dinucleotide binding

PMID:2319593[2]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

enables

GO:0015046

rubredoxin-NADP reductase activity

PMID:2319593[2]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

enables

GO:0016491

oxidoreductase activity

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR016156
InterPro:IPR023753

F

Seeded From UniProt

complete

enables

GO:0050660

flavin adenine dinucleotide binding

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR016156

F

Seeded From UniProt

complete

involved_in

GO:0055114

oxidation-reduction process

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR016156
InterPro:IPR023753

P

Seeded From UniProt

complete

enables

GO:0015044

rubredoxin-NAD+ reductase activity

GO_REF:0000003

ECO:0000501

evidence used in automatic assertion

EC:1.18.1.1

F

Seeded From UniProt

complete

enables

GO:0016491

oxidoreductase activity

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0560

F

Seeded From UniProt

complete

part_of

GO:0005737

cytoplasm

GO_REF:0000037
GO_REF:0000039

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0963
UniProtKB-SubCell:SL-0086

C

Seeded From UniProt

complete

involved_in

GO:0055114

oxidation-reduction process

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0560

P

Seeded From UniProt

complete

involved_in

GO:0043448

alkane catabolic process

GO_REF:0000041

ECO:0000322

imported manually asserted information used in automatic assertion

UniPathway:UPA00191

P

Seeded From UniProt

complete

Notes

References

See Help:References for how to manage references in GONUTS.

  1. Staijen, IE et al. (2000) Expression, stability and performance of the three-component alkane mono-oxygenase of Pseudomonas oleovorans in Escherichia coli. Eur. J. Biochem. 267 1957-65 PubMed GONUTS page
  2. 2.0 2.1 Eggink, G et al. (1990) Rubredoxin reductase of Pseudomonas oleovorans. Structural relationship to other flavoprotein oxidoreductases based on one NAD and two FAD fingerprints. J. Mol. Biol. 212 135-42 PubMed GONUTS page