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User:Entzabig

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SUPER BOWL (Round 5)

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableARATH:PLDD12012-04-09 18:35:52 CDTGO:0004630 phospholipase D activity (F)PMID:11706190ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 5- Shows phospholipase activity levels in WT and mutated gene

challenge
acceptableECOLI:TRXB2012-04-09 18:56:09 CDTGO:0004791 thioredoxin-disulfide reductase activity (F)PMID:14245401ECO:0000314 direct assay evidence used in manual assertion

Figure 13 shows the reducing ability of thioredoxin reductase.

challenge
acceptableECOLI:XYLA2012-04-09 19:05:09 CDTGO:0009045 xylose isomerase activity (F)PMID:6344793ECO:0000315 mutant phenotype evidence used in manual assertion

Table 1 shows D-xylose isomerase activity in both mutant and WT strains of E. coli.

challenge
acceptablePSECO:B1AAM32012-04-09 19:11:25 CDTGO:0050587 chlorite O2-lyase activity (F)PMID:19228194ECO:0000314 direct assay evidence used in manual assertion

Figure 3 shows the activity level of conversion from chlorite to chloride and oxygen by the enzyme.

challenge
requireschangesARATH:Q56XD42012-04-09 19:21:33 CDTGO:0047617 acyl-CoA hydrolase activity (F)PMID:14660652ECO:0000314 direct assay evidence used in manual assertion

Figure 4 shows the effects of salt on acyl-CoA thioesterase activity.

Figure 5 shows the kinetics based on two different chain lengths in the substrate.

challenge
acceptableHUMAN:ST1A32012-04-10 11:40:08 CDTGO:0004062 aryl sulfotransferase activity (F)PMID:9855620ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3 compares the dopamine sulfotransferase activity for SULT1A3 wild type and mutants.

challenge
requireschangesARATH:ALDO42012-04-12 10:35:15 CDTGO:0004031 aldehyde oxidase activity (F)PMID:19297586ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 5 shows the mutations in the enzyme that results in less conversion of BD to BA (benzaldehyde to benzoic acid).

challenge
requireschanges?2012-04-12 10:47:57 CDTGO:0004467 long-chain fatty acid-CoA ligase activity (Figure 3 shows the activity level of the enzyme, and Figure 4 shows the different levels of activity based on different substrates.)PMID:12177484IDA: Inferred from Direct Assay
challenge

acceptable:5
unacceptable:0
requires_changes:3
flagged:0

Annotations challenged by Entzabig

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
requireschangesHildeb22,
Team Payola
ECOBD:C6EFE6GO:0008184 - glycogen phosphorylase activity (F)PMID:16816199ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 1

0 annotations fixed by Entzabig


MSU Internal

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableHUMAN:PH4H2012-03-29 11:06:25 CDTGO:0004505 phenylalanine 4-monooxygenase activity (F)PMID:20667834ECO:0000314 direct assay evidence used in manual assertion

Figure 1- Shows measurements of PAH activity based on different levels of substrate and cofactors.

challenge
requireschangesECOLI:AAT2012-03-29 12:22:53 CDTGO:0047319 aspartate-phenylpyruvate transaminase activity (F)PMID:352693ECO:0000314 direct assay evidence used in manual assertion

Figure 1 shows increasing phenylalanine aminotransferase activity with increasing levels of enzyme.

challenge

acceptable:1
unacceptable:0
requires_changes:1
flagged:0

Annotations challenged by Entzabig

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
requireschangesHildeb22,
Team Payola
ECOBD:C6EFE6GO:0008184 - glycogen phosphorylase activity (F)PMID:16816199ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 1

0 annotations fixed by Entzabig