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PMID:352693

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Citation

Powell, JT and Morrison, JF (1978) The purification and properties of the aspartate aminotransferase and aromatic-amino-acid aminotransferase from Escherichia coli. Eur. J. Biochem. 87:391-400

Abstract

A simple and convenient procedure is described for the isolation in good yield of two amino-transferases from various strains of Escherichia coli. On the basis of their substrate specificities one of the enzymes has been classified as an aromatic amino acid aminotransferase and the other as an aspartate aminotransferase, but both act on a wide range of substrates. Pyridoxal phosphate is bound more strongly to the aspartate aminotransferase than to the aromatic amino transferase which cannot be fully re-activated after removal of the prosthetic group. Both enzymes are composed of two subunits which appear to be identical.

Links

PubMed

Keywords

Amino Acids/analysis; Aspartate Aminotransferases/isolation & purification; Aspartate Aminotransferases/metabolism; Escherichia coli/enzymology; Kinetics; Macromolecular Substances; Molecular Weight; Species Specificity; Substrate Specificity; Transaminases/isolation & purification; Transaminases/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:AAT

located_in

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ECOLI:TYRB

located_in

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ECOLI:AAT

GO:0047319: aspartate-phenylpyruvate transaminase activity

ECO:0000314:

F

Figure 1 shows increasing phenylalanine aminotransferase activity with increasing levels of enzyme.

complete
CACAO 4071

ECOLI:AAT

part_of

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ECOLI:AAT

enables

GO:0030170: pyridoxal phosphate binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ECOLI:TYRB

part_of

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ECOLI:TYRB

enables

GO:0030170: pyridoxal phosphate binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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