PMID:12177484

Citation	Shockey, JM, Fulda, MS and Browse, JA (2002) Arabidopsis contains nine long-chain acyl-coenzyme a synthetase genes that participate in fatty acid and glycerolipid metabolism. Plant Physiol. 129:1710-22
Abstract	Long-chain acyl-coenzyme A (CoA) synthetases (LACSs) activate free fatty acids to acyl-CoA thioesters and as such play critical roles in fatty acid metabolism. This important class of enzymes factors prominently in several fatty acid-derived metabolic pathways, including phospholipid, triacylglycerol, and jasmonate biosynthesis and fatty acid beta-oxidation. In an effort to better understand the factors that control fatty acid metabolism in oilseeds, we have sought to identify and characterize genes that encode LACSs in Arabidopsis. Nine cDNAs were identified, cloned, and tested for their ability to complement a LACS-deficient strain of yeast (Saccharomyces cerevisiae). Seven of the nine successfully restored growth, whereas two cDNAs encoding putative peroxisomal isoforms did not. Lysates from yeast cells overexpressing each of the nine cDNAs were active in LACS enzyme assays using oleic acid as a substrate. The substrate specificities of the enzymes were determined after overexpression in LACS-deficient Escherichia coli. Most of the LACS enzymes displayed highest levels of activity with the fatty acids that make up the common structural and storage lipids in Arabidopsis tissues. Analysis of the tissue-specific expression profiles for these genes revealed one flower-specific isoform, whereas all others were expressed in various tissues throughout the plant. These nine cDNAs are thought to constitute the entire LACS family in Arabidopsis, and as such, will serve as powerful tools in the study of acyl-CoA metabolism in oilseeds.
Links	PubMed PMC166759 Online version:10.1104/pp.003269
Keywords	Amino Acid Sequence; Arabidopsis/enzymology; Arabidopsis/genetics; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Cloning, Molecular; Coenzyme A Ligases/genetics; Coenzyme A Ligases/metabolism; Fatty Acids/metabolism; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Genetic Complementation Test; Glycerides/metabolism; Isoenzymes/genetics; Isoenzymes/metabolism; Molecular Sequence Data; Multigene Family/genetics; Mutation; Phylogeny; Saccharomyces cerevisiae/genetics; Sequence Homology, Amino Acid; Substrate Specificity
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